Perinatal exposure to organohalogen pollutants decreases vasopressin content and its mRNA expression in magnocellular neuroendocrine cells activated by osmotic stress in adult rats
Autor: | Francisco Pellicer, Edith Sánchez-Jaramillo, Margarita C. Currás-Collazo, Mhar Y. Alvarez-Gonzalez, Víctor Rivelino Juárez-González, L.E. Orser, Martha León-Olea, Prasada Rao S. Kodavanti, Samuel Mucio-Ramírez, Eduardo Sánchez-Islas, Borin Hou |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male Vasopressin Transcription Genetic Messenger Wistar Sodium Chloride Toxicology Rats Sprague-Dawley 0302 clinical medicine Polybrominated diphenyl ethers Pregnancy Gene expression Halogenated Diphenyl Ethers PCBs Salt loading Pharmacology and Pharmaceutical Sciences Chlorodiphenyl (54% Chlorine) Maternal Exposure Prenatal Exposure Delayed Effects Environmental Pollutants Female Supraoptic Nucleus Proto-Oncogene Proteins c-fos Transcription hormones hormone substitutes and hormone antagonists medicine.medical_specialty endocrine system Osmotic shock PBDEs Down-Regulation In situ hybridization Biology Article 03 medical and health sciences Neuroendocrine disruption Genetic Neuroendocrine Cells Osmotic Pressure Internal medicine medicine Animals RNA Messenger Rats Wistar Pharmacology Messenger RNA Osmotic concentration Neurotoxicity Neurosciences medicine.disease Rats Arginine Vasopressin cFOS 030104 developmental biology Endocrinology nervous system Chlorodiphenyl RNA Sprague-Dawley 030217 neurology & neurosurgery Paraventricular Hypothalamic Nucleus |
Zdroj: | Toxicology and applied pharmacology, vol 329 |
Popis: | Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are environmental pollutants that produce neurotoxicity and neuroendocrine disruption. They affect the vasopressinergic system but their disruptive mechanisms are not well understood. Our group reported that rats perinatally exposed to Aroclor-1254 (A1254) and DE-71 (commercial mixtures of PCBs and PBDEs) decrease somatodendritic vasopressin (AVP) release while increasing plasma AVP responses to osmotic activation, potentially emptying AVP reserves required for body-water balance. The aim of this research was to evaluate the effects of perinatal exposure to A1254 or DE-71 (30 mg kg/day) on AVP transcription and protein content in the paraventricular and supraoptic hypothalamic nuclei, of male and female rats, by in situ hybridization and immunohistochemistry. cFOS mRNA expression was evaluated in order to determine neuroendocrine cells activation due to osmotic stimulation. Animal groups were: vehicle (control); exposed to either A1254 or DE-71; both, control and exposed, subjected to osmotic challenge. The results confirmed a physiological increase in AVP-immunoreactivity (AVP-IR) and gene expression in response to osmotic challenge as reported elsewhere. In contrast, the exposed groups did not show this response to osmotic activation, they showed significant reduction in AVP-IR neurons, and AVP mRNA expression as compared to the hyperosmotic controls. cFOS mRNA expression increased in A1254 dehydrated groups, suggesting that the AVP-IR decrease was not due to a lack of the response to the osmotic activation. Therefore, A1254 may interfere with the activation of AVP mRNA transcript levels and protein, causing a central dysfunction of vasopressinergic system. |
Databáze: | OpenAIRE |
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