Perinatal exposure to organohalogen pollutants decreases vasopressin content and its mRNA expression in magnocellular neuroendocrine cells activated by osmotic stress in adult rats

Autor: Francisco Pellicer, Edith Sánchez-Jaramillo, Margarita C. Currás-Collazo, Mhar Y. Alvarez-Gonzalez, Víctor Rivelino Juárez-González, L.E. Orser, Martha León-Olea, Prasada Rao S. Kodavanti, Samuel Mucio-Ramírez, Eduardo Sánchez-Islas, Borin Hou
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Vasopressin
Transcription
Genetic
Messenger
Wistar
Sodium Chloride
Toxicology
Rats
Sprague-Dawley
0302 clinical medicine
Polybrominated diphenyl ethers
Pregnancy
Gene expression
Halogenated Diphenyl Ethers
PCBs
Salt loading
Pharmacology and Pharmaceutical Sciences
Chlorodiphenyl (54% Chlorine)
Maternal Exposure
Prenatal Exposure Delayed Effects
Environmental Pollutants
Female
Supraoptic Nucleus
Proto-Oncogene Proteins c-fos
Transcription
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
endocrine system
Osmotic shock
PBDEs
Down-Regulation
In situ hybridization
Biology
Article
03 medical and health sciences
Neuroendocrine disruption
Genetic
Neuroendocrine Cells
Osmotic Pressure
Internal medicine
medicine
Animals
RNA
Messenger
Rats
Wistar
Pharmacology
Messenger RNA
Osmotic concentration
Neurotoxicity
Neurosciences
medicine.disease
Rats
Arginine Vasopressin
cFOS
030104 developmental biology
Endocrinology
nervous system
Chlorodiphenyl
RNA
Sprague-Dawley
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
Zdroj: Toxicology and applied pharmacology, vol 329
Popis: Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are environmental pollutants that produce neurotoxicity and neuroendocrine disruption. They affect the vasopressinergic system but their disruptive mechanisms are not well understood. Our group reported that rats perinatally exposed to Aroclor-1254 (A1254) and DE-71 (commercial mixtures of PCBs and PBDEs) decrease somatodendritic vasopressin (AVP) release while increasing plasma AVP responses to osmotic activation, potentially emptying AVP reserves required for body-water balance. The aim of this research was to evaluate the effects of perinatal exposure to A1254 or DE-71 (30 mg kg/day) on AVP transcription and protein content in the paraventricular and supraoptic hypothalamic nuclei, of male and female rats, by in situ hybridization and immunohistochemistry. cFOS mRNA expression was evaluated in order to determine neuroendocrine cells activation due to osmotic stimulation. Animal groups were: vehicle (control); exposed to either A1254 or DE-71; both, control and exposed, subjected to osmotic challenge. The results confirmed a physiological increase in AVP-immunoreactivity (AVP-IR) and gene expression in response to osmotic challenge as reported elsewhere. In contrast, the exposed groups did not show this response to osmotic activation, they showed significant reduction in AVP-IR neurons, and AVP mRNA expression as compared to the hyperosmotic controls. cFOS mRNA expression increased in A1254 dehydrated groups, suggesting that the AVP-IR decrease was not due to a lack of the response to the osmotic activation. Therefore, A1254 may interfere with the activation of AVP mRNA transcript levels and protein, causing a central dysfunction of vasopressinergic system.
Databáze: OpenAIRE
Externí odkaz: