Maternal nutrient restriction in mid-to-late gestation influences fetal mRNA expression in muscle tissues in beef cattle

Autor: Stephen P. Miller, Kendall C Swanson, Brian W. McBride, K. M. Wood, Carolyn Fitzsimmons, Francois Paradis
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
lcsh:QH426-470
muscle
medicine.medical_treatment
lcsh:Biotechnology
liver
03 medical and health sciences
Insulin-like growth factor
Fetus
Pregnancy
Internal medicine
lcsh:TP248.13-248.65
Gene expression
Genetics
medicine
Animals
RNA
Messenger
Myogenin
Insulin-like growth factor 1 receptor
nutrient restriction
microRNA
biology
Gene Expression Profiling
Muscles
Insulin-like growth factor 2 receptor
0402 animal and dairy science
IGF2
Beef cattle
Maternal Nutritional Physiological Phenomena
04 agricultural and veterinary sciences
DNA Methylation
040201 dairy & animal science
Red Meat
Insulin receptor
lcsh:Genetics
Phenotype
030104 developmental biology
Endocrinology
fetal programming
Insulin-like growth factor 2
DNA methylation
biology.protein
Cattle
Female
methylation
Research Article
Biotechnology
Zdroj: BMC Genomics, Vol 18, Iss 1, Pp 1-14 (2017)
BMC Genomics
ISSN: 1471-2164
DOI: 10.1186/s12864-017-4051-5
Popis: Background Manipulating maternal nutrition during specific periods of gestation can result in re-programming of fetal and post-natal development. In this experiment we investigated how a feed restriction of 85% compared with 140% of total metabolizable energy requirements, fed to cows during mid-to-late gestation, influences phenotypic development of fetuses and mRNA expression of growth (Insulin-Like Growth Factor family and Insulin Receptor (INSR)), myogenic (Myogenic Differentiation 1 (MYOD1), Myogenin (MYOG), Myocyte Enhancer Factor 2A (MEF2A), Serum Response Factor (SRF)) and adipogenic (Peroxisome Proliferator Activated Receptor Gamma (PPARG)) genes in fetal longissimus dorsi (LD) and semitendinosus (ST) muscle. DNA methylation of imprinted genes, Insulin Like Growth Factor 2 (IGF2) and Insulin Like Growth Factor 2 Receptor (IGF2R), and micro RNA (miRNA) expression, were also examined as potential consequences of poor maternal nutrition, but also potential regulators of altered gene expression patterns. Results While the nutrient restriction impacted dam body weight, no differences were observed in phenotypic fetal measurements (weight, crown-rump length, or thorax circumference). Interestingly, LD and ST muscles responded differently to the differential pre-natal nutrient levels. While LD muscle of restricted fetal calves had greater mRNA abundances for Insulin Like Growth Factor 1 and its receptor (IGF1 and IGF1R), IGF2R, INSR, MYOD1, MYOG, and PPARG, no significant differences were observed for gene expression in ST muscle. Similarly, feed restriction had a greater impact on the methylation level of IGF2 Differentially Methylated Region 2 (DMR2) in LD muscle as compared to ST muscle between treatment groups. A negative correlation existed between IGF2 mRNA expression and IGF2 DMR2 methylation level in both LD and ST muscles. Differential expression of miRNAs 1 and 133a were also detected in LD muscle. Conclusions Our data suggests that a nutrient restriction of 85% as compared to 140% of total metabolizable energy requirements during the 2nd half of gestation can alter the expression of growth, myogenic and adipogenic genes in fetal muscle without apparent differences in fetal phenotype. It also appears that the impact of feed restriction varies between muscles suggesting a priority for nutrient partitioning depending on muscle function and/or fiber composition. Differences in the methylation level in IGF2, a well-known imprinted gene, as well as differences in miRNA expression, may be functional mechanisms that precede the differences in gene expression observed, and could lead to trans-generational epigenetic programming.
Databáze: OpenAIRE
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