Cell-cell communication in sourdough lactic acid bacteria: a proteomic study in Lactobacillus sanfranciscensis CB1

Autor: Raffaella Di Cagno, Maria Carmela Simonetti, Maria De Angelis, Solange Buchin, Fabio Minervini, Antonio Limitone, Marco Gobbetti
Přispěvatelé: Università degli Studi di Bari Aldo Moro, Unité de recherches en Technologie et Analyses Laitières (URTAL), Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Proteomics
Spectrometry
Mass
Electrospray Ionization
Cell
Lactobacillus sanfranciscensis
Biology
Biochemistry
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Tandem Mass Spectrometry
medicine
Electrophoresis
Gel
Two-Dimensional
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Lactic Acid
Furans
Molecular Biology
Gene
Phylogeny
MICROBIAL INTERACTION
030304 developmental biology
0303 health sciences
Microbial Viability
Base Sequence
030306 microbiology
Lactobacillus brevis
CELL-CELL COMMUNICATION
food and beverages
Lactobacillaceae
Bread
Gene Expression Regulation
Bacterial
biology.organism_classification
Lactic acid
SOURDOUGH LACTIC ACID BACTERIA
Kinetics
Lactobacillus
medicine.anatomical_structure
Phenotype
chemistry
LUXS
Lactobacillus plantarum
Bacteria
Chromatography
Liquid
Hydrogen
Zdroj: Proteomics
Proteomics, Wiley-VCH Verlag, 2007, 7 (14), pp.2430-2446. ⟨10.1002/pmic.200700143⟩
ISSN: 1615-9853
1615-9861
DOI: 10.1002/pmic.200700143⟩
Popis: International audience; The mechanisms of cell-cell communication in Lactobacillus sanfranciscensis CB1 were studied. The highest number of dead/damaged cells of L. sanfranciscensis CB1 was found in cocultures with Lactobacillus plantarum DC400 or Lactobacillus brevis CR13 when the late stationary phase of growth (18 h) was reached. 2-DE analysis was carried out. Almost the same proteins were induced in all three cocultures at the mid-exponential phase of growth (7 h). The number of induced proteins markedly increased at 18 h, especially when L. sanfranciscensis CB1 was cocultured with L. plantarum DC400 or L. brevis CR13. Nineteen overexpressed proteins were identified. These proteins had a central role in stress response mechanisms and LuxS-mediated signalling was involved in the regulation of most of them. The luxS and metF genes were partially sequenced in L. sanfranciscensis CB1. RT-PCR showed that the expression of luxS gene decreased from 7 to 12 h. It was highest in cocultures with L. plantarum DC400 and L. brevis CR13. 2(3H)dihydrofuranone-5ethyl and 2(3H)dihydrofuranone-5pentyl were identified as presumptive signalling molecules when L. sanfranciscensis CB1 was cocultured with L. brevis CR13 and, especially, L. plantarum DC400. The synthesis of other volatile compounds and peptidase activities were also influenced by the type of microbial cocultures.
Databáze: OpenAIRE
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