Response of Human Chondrocytes Prepared for Autologous Implantation to Growth Factors
| Autor: | Arnavaz A. Hakimiyan, L. Rappoport, Brian J. Cole, David C. Rueger, Susan Chubinskaya, Adam B. Yanke |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Cartilage Articular Anabolism Decorin Bone Morphogenetic Protein 7 Matrix (biology) Transplantation Autologous Chondrocyte Chondrocytes Transforming Growth Factor beta medicine Humans Orthopedics and Sports Medicine Insulin-Like Growth Factor I Autologous chondrocyte implantation Cells Cultured Aggrecan biology business.industry Histology Molecular biology Fibronectin medicine.anatomical_structure Bone Morphogenetic Proteins Immunology biology.protein Intercellular Signaling Peptides and Proteins Surgery business |
| Zdroj: | Journal of Knee Surgery. 21:192-199 |
| ISSN: | 1938-2480 1538-8506 |
| DOI: | 10.1055/s-0030-1247818 |
| Popis: | This study investigated metabolism of autologous chondrocytes after initial expansion imme- diately before implantation. Chondrocytes cultured in either monolayers or alginate beads were treated with insulin-like growth factor-1 (IGF-1), osteogenic pro- tein-1 (OP-1), or a combination. Proteoglycan synthesis and DNA content were tested in both cultures. Alginate beads also were analyzed with live/dead cell assay, saf- ranin O/fast green stain for histology, and immunohis- tochemistry with antibodies against collagen type II and VI, aggrecan, decorin, and fibronectin. In monolayers, autologous chondrocytes changed their morphologic appearance. In alginate, they maintained chondrocytic phenotype. Growth factors, especially combined, pro- moted cell survival and induced chondrocyte prolifera- tion. OP-1 stimulated the largest cartilage-specific ma - trix and the most accumulation of collagen type II and fibronectin, although the overall matrix synthesized by autologous chondrocyte implantation cells was smaller than that produced by normal chondrocytes. The clini- cal implications of this study suggest a significant prom - ise for anabolic growth factors in cartilage repair as a potential modifying therapy for the enhancement of chondrocytic phenotype of autologous chondrocytes. |
| Databáze: | OpenAIRE |
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