Evaluation of Polymorphic Locus Sequence Typing for Candida glabrata Epidemiology
| Autor: | Thomas D. Edlind, Kelley R. Healey, Santosh K. Katiyar, John-Paul Vermitsky, Celeste Shelton, Eric Shiffrin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genotype Epidemiology 030106 microbiology Candida glabrata Mycological Typing Techniques 03 medical and health sciences Genetic variation Pulsed-field gel electrophoresis Cluster Analysis Humans Typing Genetics Molecular Epidemiology Molecular epidemiology biology Candidiasis Genetic Variation biology.organism_classification 030104 developmental biology Genetic Loci Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | Journal of Clinical Microbiology. 54:1042-1050 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The opportunistic yeast Candida glabrata is increasingly refractory to antifungal treatment or prophylaxis and relatedly is increasingly implicated in health care-associated infections. To elucidate the epidemiology of these infections, strain typing is required. Sequence-based typing provides multiple advantages over length-based methods, such as pulsed-field gel electrophoresis (PFGE); however, conventional multilocus sequence typing (targeting 6 conserved loci) and whole-genome sequencing are impractical for routine use. A commercial sequence-based typing service for C. glabrata that targets polymorphic tandem repeat-containing loci has recently been developed. These CgMT-J and CgMT-M services were evaluated with 56 epidemiologically unrelated isolates, 4 to 7 fluconazole-susceptible or fluconazole-resistant isolates from each of 5 center A patients, 5 matched pairs of fluconazole-susceptible/resistant isolates from center B patients, and 7 isolates from a center C patient who responded to then failed caspofungin therapy. CgMT-J and CgMT-M generated congruent results, resolving isolates into 24 and 20 alleles, respectively. Isolates from all but one of the center A patients shared the same otherwise rare alleles, suggesting nosocomial transmission. Unexpectedly, Pdr1 sequencing showed that resistance arose independently in each patient. Similarly, most isolates from center B also clustered together; however, this may reflect a dominant clone since their alleles were shared by multiple unrelated isolates. Although distinguishable by their echinocandin susceptibilities, all isolates from the center C patient shared alleles, in agreement with the previously reported relatedness of these isolates based on PFGE. Finally, we show how phylogenetic clusters can be used to provide surrogate parents to analyze the mutational basis for antifungal resistance. |
| Databáze: | OpenAIRE |
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