Dietary nitrate does not reduce oxygen cost of exercise or improve muscle mitochondrial function in patients with mitochondrial myopathy
| Autor: | Klaas Nicolay, Jeanine J. Prompers, Irenaeus F.M. de Coo, Carlijn M. P. le Clercq, Natal A. W. van Riel, Luc J. C. van Loon, Hubert J.M. Smeets, Joep P. J. Schmitz, Miranda Nabben, Stephan F. E. Praet, Harm R. Haak, Jolita Ciapaite |
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| Přispěvatelé: | ACS - Microcirculation, Amsterdam Gastroenterology Endocrinology Metabolism, Experimental Vascular Medicine, Neurology, Rehabilitation Medicine, Moleculaire Genetica, RS: CARIM - R2.06 - Intermediate cardiac metabolism, RS: CAPHRI - R1 - Ageing and Long-Term Care, Interne Geneeskunde, RS: FHML MaCSBio, RS: GROW - R4 - Reproductive and Perinatal Medicine, RS: CARIM - R2.10 - Mitochondrial disease, Klinische Genetica, Bewegingswetenschappen, RS: NUTRIM - R3 - Respiratory & Age-related Health, RS: NUTRIM - HB/BW section A, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, Computational Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male P-magnetic resonance spectroscopy Mitochondrial Myopathies/drug therapy Physiology Administration Oral Muscle Strength/drug effects Nitrate Oxygen SUPPLEMENTATION Oxygen Consumption/drug effects chemistry.chemical_compound 0302 clinical medicine Mitochondrial myopathy Exercise Tolerance/drug effects Exercise Tolerance Permeabilized muscle fibers Mitochondrial Myopathies HUMAN SKELETAL-MUSCLE Middle Aged Nitrates/administration & dosage Mitochondria Treatment Outcome RESPIRATION Administration INORGANIC NITRATE Female medicine.symptom P-31-magnetic resonance spectroscopy Oral Adult medicine.medical_specialty EFFICIENCY VO chemistry.chemical_element Exercise intolerance Biology V̇o2peak Nitric oxide 03 medical and health sciences Young Adult Oxygen Consumption Physiology (medical) Internal medicine 31P-magnetic resonance spectroscopy Dietary Nitrate Respiration medicine Humans In patient O-2 COST Muscle/drug effects Muscle Strength Exercise Aged Nitrates NITRIC-OXIDE Muscle weakness (V) over dot(O2peak) CONSUMPTION medicine.disease Mitochondria Muscle Mitochondria Muscle/drug effects 030104 developmental biology Endocrinology chemistry TIME-TRIAL PERFORMANCE Physical therapy Psychomotor Performance/drug effects Psychomotor Performance 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 312(5), R689-R701. American Physiological Society American Journal of Physiology-Regulatory Integrative and Comparative Physi, 312(5), R689-R701. American Physiological Society American Journal of Physiology-regulatory Integrative and Comparative Physiology, 312(5), R689-R701. American Physiological Society American Journal of Physiology : Regulatory Integrative and Comparative Physiology, 312(5), R689-R701. American Physiological Society |
| ISSN: | 1522-1490 0363-6119 |
| Popis: | Muscle weakness and exercise intolerance negatively affect the quality of life of patients with mitochondrial myopathy. Short-term dietary nitrate supplementation has been shown to improve exercise performance and reduce oxygen cost of exercise in healthy humans and trained athletes. We investigated whether 1 wk of dietary inorganic nitrate supplementation decreases the oxygen cost of exercise and improves mitochondrial function in patients with mitochondrial myopathy. Ten patients with mitochondrial myopathy (40 ± 5 yr, maximal whole body oxygen uptake = 21.2 ± 3.2 ml·min−1·kg body wt−1, maximal work load = 122 ± 26 W) received 8.5 mg·kg body wt−1·day−1inorganic nitrate (~7 mmol) for 8 days. Whole body oxygen consumption at 50% of the maximal work load, in vivo skeletal muscle oxidative capacity (evaluated from postexercise phosphocreatine recovery using31P-magnetic resonance spectroscopy), and ex vivo mitochondrial oxidative capacity in permeabilized skinned muscle fibers (measured with high-resolution respirometry) were determined before and after nitrate supplementation. Despite a sixfold increase in plasma nitrate levels, nitrate supplementation did not affect whole body oxygen cost during submaximal exercise. Additionally, no beneficial effects of nitrate were found on in vivo or ex vivo muscle mitochondrial oxidative capacity. This is the first time that the therapeutic potential of dietary nitrate for patients with mitochondrial myopathy was evaluated. We conclude that 1 wk of dietary nitrate supplementation does not reduce oxygen cost of exercise or improve mitochondrial function in the group of patients tested. |
| Databáze: | OpenAIRE |
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