Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection
| Autor: | Sean M. Grimmond, James C. Smith, Joshua M. Brickman, Rosa S.P. Beddington, Alistair R. R. Forrest, Melanie Clements, Amer Ahmed Rana, Sally L. Dunwoodie, Rita Sousa-Nunes, Ross Kettleborough, Philip Avner |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Sequence analysis Biology Mice Complementary DNA Genetics medicine Animals Letters Gene Crosses Genetic Genetics (clinical) Gene Library Regulation of gene expression Gene Expression Profiling Endoderm Gene Expression Regulation Developmental Sequence Analysis DNA Molecular biology Housekeeping gene Cell biology Mice Inbred C57BL Gene expression profiling medicine.anatomical_structure embryonic structures Mice Inbred CBA Definitive endoderm |
| Zdroj: | Genome Research. 13:2609-2620 |
| ISSN: | 1088-9051 |
| Popis: | This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns. |
| Databáze: | OpenAIRE |
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