Copaifera multijuga oleoresin and its constituent diterpene (−)-copalic acid: Genotoxicity and chemoprevention study
| Autor: | Jacqueline Morais Alves, Luiza J. Carneiro, Jairo Kenupp Bastos, Daiane Eleutério Pereira, Denise Crispim Tavares, Juliana Marques Senedese, Sérgio Ricardo Ambrósio, Pâmela Tinti de Castro, Luís Fernando Leandro |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Health Toxicology and Mutagenesis Copaifera Pharmacology medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Aberrant Crypt Foci Botany Copaiba Genetics medicine Animals Anticarcinogenic Agents Oleoresin Rats Wistar Micronuclei Chromosome-Defective Dose-Response Relationship Drug biology Plant Extracts Fabaceae CARCINOGÊNESE biology.organism_classification digestive system diseases Comet assay 030104 developmental biology chemistry 030220 oncology & carcinogenesis Colonic Neoplasms Micronucleus test Hepatocytes Comet Assay Diterpenes Micronucleus Genotoxicity DNA Damage Aberrant crypt foci |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2017.05.001 |
| Popis: | Copaiba oleoresins are used in alternative medicine as anti-inflammatory, antitumoral, and antimicrobial treatments. (-)-Copalic acid (CA) is the major diterpene found in exudates from Copaifera species. We have examined the genotoxicity and the chemopreventive potential of Copaifera multijuga oleoresin (CM) and CA. Genotoxicity assessment was examined with the peripheral blood micronucleus test and the comet assay (male Swiss mouse hepatocytes). In the chemoprevention study, we evaluated the effects of CM and CA on the formation of 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) in male Wistar rat colon. Neither agent caused a significant increase in micronucleus frequency relative to controls, but the highest CM dose tested (400mg/kg b.w.) caused DNA damage in the comet assay. Both agents significantly reduced the frequency of DMH-induced ACF. Both CM and CA suppressed ACF formation and may have a protective effect against colon carcinogenesis. |
| Databáze: | OpenAIRE |
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