Stability of Direct Oral Anticoagulants and Antiarrhythmic Drugs in Serum Collected in Standard (Nongel) Serum Tubes Versus Tubes Containing Gel Separators
| Autor: | Ole-Martin Fuskevåg, Olav Spigset, Siri Amundsen, Solfrid Hegstad, Arne Helland, Marina Gule |
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| Rok vydání: | 2022 |
| Předmět: |
Pharmacology
Blood Specimen Collection Rivaroxaban Chromatography medicine.diagnostic_test Chemistry Anticoagulants Serum concentration chemistry.chemical_compound Adsorption Therapeutic drug monitoring Edoxaban medicine Humans Pharmacology (medical) Ultra high pressure Vacutainer Anti-Arrhythmia Agents Gels Volume concentration Chromatography Liquid medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring. 44:328-334 |
| ISSN: | 0163-4356 |
| Popis: | Background Separation gels are often used in collection tubes, but adsorption of drugs onto the gel may cause falsely low concentrations in therapeutic drug monitoring. In this study, the stability of apixaban, edoxaban, rivaroxaban, flecainide, amiodarone, and desethylamiodarone was assessed in tubes, with and without gel separators. Methods Drug-free blood was spiked and stored for up to 7 d in non-gel tubes and gel tubes from two manufacturers (Vacuette and Vacutainer). The samples were analyzed in triplicates using ultra high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Results At ambient temperature conditions, the serum concentrations of apixaban, edoxaban, and rivaroxaban in a tube with acrylic-based gel had already decreased at baseline, while it took 6 h to observe the same result in a tube with olefin-based gel. At 4 °C, the reduction in serum concentration was considerably slower. For flecainide, the gel tube concentrations were stable at ambient temperature for 3 d, but decreased after 7 d in acrylic-based gel tubes. Amiodarone and desethylamiodarone stored in gel tubes at 4 °C showed decrease in concentrations after 24 h and 6 h, respectively. Conclusion Acrylic-based gel tubes should not be used for any of the tested drugs. Although olefin-based gel tubes may be used for anticoagulants and flecainide, it is advisable to prefer non-gel tubes as a general precaution. |
| Databáze: | OpenAIRE |
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