Sperm Penetration Assay as an Indicator of Bull Fertility
| Autor: | Myung-Geol Pang, Woo-Sung Kwon, Kichoon Lee, Shin-Ae Oh, El-Sayed A. Mohamed, Sung-Jae Yoon, Yoo-Jin Park, Heung-Ruil Kim, Myeung-Sik Lee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system media_common.quotation_subject Hamster Fertility Biology Sensitivity and Specificity Severity of Illness Index Sperm penetration Fertility index Andrology Cricetinae Animals Cells Cultured Infertility Male reproductive and urinary physiology Normal range media_common Cryopreservation Sperm-Ovum Interactions Mesocricetus Heparin urogenital system Osmolar Concentration Reproducibility of Results Spermatozoa Sperm Coculture Techniques Semen Analysis Male fertility Oocytes Cattle Female Animal Science and Zoology Sperm Capacitation Animals Inbred Strains Semen Preservation |
| Zdroj: | Journal of Reproduction and Development. 58:461-466 |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pre-incubated and coincubated with oocytes in media containing various concentrations of heparin (0 to 50 μg/ml). Coincubation with 10 μg/ml heparin showed the highest sperm penetration (P |
| Databáze: | OpenAIRE |
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