Hypoxanthine Phosphoribosyl Transferase 1 Is Upregulated, Predicts Clinical Outcome and Controls Gene Expression in Breast Cancer
| Autor: | Ramadevi Subramani, Enrique I Ramos, Ramesh Choudhari, Shrikanth S. Gadad, Rajkumar Lakshmanaswamy, Alana L. Harrison, Melina Sedano, Mina Zilaie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research endocrine system HPRT1 Biology lcsh:RC254-282 Article Transcriptome 03 medical and health sciences 0302 clinical medicine Breast cancer breast cancer stomatognathic system Gene expression medicine genomics cancer heterocyclic compounds Gene Gene knockdown Cancer medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Housekeeping gene enzymes and coenzymes (carbohydrates) 030104 developmental biology Oncology Hypoxanthine-guanine phosphoribosyltransferase 030220 oncology & carcinogenesis Cancer research |
| Zdroj: | Cancers, Vol 12, Iss 1522, p 1522 (2020) Cancers Volume 12 Issue 6 |
| ISSN: | 2072-6694 |
| Popis: | Hypoxanthine phosphoribosyl transferase 1 (HPRT1) is traditionally believed to be a housekeeping gene however, recent reports suggest that it is upregulated in several cancers and is associated with clinical outcomes. HPRT1 is located on chromosome X and encodes the HPRT enzyme, which functions in recycling nucleotides to supply for DNA and RNA synthesis in actively dividing cells. Here, we used transcriptomic analyses to interrogate its expression across all known cancer types and elucidated its role in regulating gene expression in breast cancer. We observed elevated HPRT1 RNA levels in malignant tissues when compared to normal controls, indicating its potential as a diagnostic and prognostic marker. Further, in breast cancer, the subtype-specific analysis showed that its expression was highest in basal and triple-negative breast cancer, and HPRT1 knockdown in breast cancer cells suggested that HPRT1 positively regulates genes related to cancer pathways. Collectively, our results essentially highlight the importance of and change the way in which HPRT1&rsquo s function is studied in biology, warranting careful examination of its role in cancer. |
| Databáze: | OpenAIRE |
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