Effect of Nutrients on Defined Bacterial Plaques and Streptococcus mutans C67-1 Implantation in a Model Mouth
| Autor: | C. J. Perrons, Helen D. Donoghue |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Sucrose
Saliva Colony Count Microbial Dental Plaque Colonisation resistance Bacterial growth Dental plaque Models Biological Microbiology Streptococcus mutans Veillonella stomatognathic system Antibiosis medicine Actinomyces Humans Salivary Proteins and Peptides General Dentistry Veillonella dispar Glycoproteins Ecology biology Biofilm Saliva Artificial Streptococcus biology.organism_classification medicine.disease Culture Media stomatognathic diseases Glucose Streptococcus oralis |
| Zdroj: | Caries Research. 25:108-115 |
| ISSN: | 1421-976X 0008-6568 |
| DOI: | 10.1159/000261352 |
| Popis: | Actinomyces viscosus WVU 627, Streptococcus oralis LPA-1 and Veillonella dispar OMZ 193 were cocultured on teeth in a model mouth for 66 h. Synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. When Streptococcus mutans C67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but there was pronounced colonization resistance. With saliva only, the proportion of S. mutans at 66 h was less than 0.5% of the total cultivable microflora. When a glucose supplement was delivered for 1 h every 6 h, S. mutans attained a final proportion of 2.4%. With sucrose, both S. mutans C67-1 and its non-cariogenic glucan-deficient mutant, C67-25, attained similar proportions of 15-20%. These experiments indicate how this model can be used to study the factors influencing colonizing ability and microbial interactions in biofilms under controlled conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|