Human eosinophil cationic protein enhances stress fiber formation in Balb/c 3T3 fibroblasts and differentiation of rat neonatal cardiomyocytes
| Autor: | Midori Kitazoe, Miki Iwata, Satoshi Hirohata, Takayuki Fukuda, Masaharu Seno, David S. Salomon, Katsuyuki Tanizawa, Takashi Maeda, Shun'ichi Kuroda |
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| Rok vydání: | 2009 |
| Předmět: |
Stress fiber
Pyridines education Clinical Biochemistry Biology Mice fluids and secretions Endocrinology medicine Animals Humans Myocytes Cardiac Enzyme Inhibitors Cytoskeleton Fibroblast Rho-associated protein kinase Actin Mice Inbred BALB C Eosinophil cationic protein Eosinophil Cationic Protein Cardiac muscle Cell Differentiation 3T3 Cells Cell Biology Amides Molecular biology Actins Rats medicine.anatomical_structure Stress Mechanical Signal transduction Signal Transduction |
| Zdroj: | Growth Factors. 27:228-236 |
| ISSN: | 1029-2292 0897-7194 |
| Popis: | We found that eosinophil cationic protein (ECP) stimulated the growth of mouse Balb/c 3T3 fibroblasts. ECP-treated 3T3 cells were more flattened and exhibited enhanced stress fiber formation. The enhancement of cytoskeleton after addition of recombinant ECP appeared stable and was able to inhibit disassembly of actin filaments that was induced by fibroblast growth factor-2. The ROCK inhibitor, Y-27632, abrogated this enhancement on stress fiber formation that was induced by ECP indicating the involvement of Rho/ROCK signaling pathway. The effect of ECP was assessed on the differentiation of primary cardiomyocytes derived from rat neonatal heart since the development of actin filaments is significantly related with organization of stress fibers. As the result, both beating rate and the expression of cardiac muscle specific markers such as atrial natriuretic factor were enhanced in the presence of ECP. Thus ECP may also function as a cardiomyocyte differentiation factor. |
| Databáze: | OpenAIRE |
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