Reactivity of 42 disulfides with thiol group of human haemoglobin and human serum albumin
| Autor: | Bernard Sebille, Jean-Pierre Mahieu, Noëlle-Martine Gosselet, Yves Beuzard, M.C. Garel |
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| Rok vydání: | 1993 |
| Předmět: |
Serum albumin
Biochemistry High-performance liquid chromatography Hemoglobins Structure-Activity Relationship Structural Biology Antisickling Agents medicine Organic chemistry Humans Reactivity (chemistry) Cysteine Disulfides Molecular Biology Serum Albumin chemistry.chemical_classification Chromatography biology Molecular Structure General Medicine Reversed-phase chromatography Human serum albumin Chromatography Ion Exchange Kinetics chemistry Drug Design Lipophilicity Thiol biology.protein medicine.drug |
| Zdroj: | International journal of biological macromolecules. 15(4) |
| ISSN: | 0141-8130 |
| Popis: | The reactivities of disulfides of different compound families towards thiol groups of human haemoglobin and human serum albumin were determined at physiological pH 7.4 by anion-exchange liquid chromatography. The apparent second-order kinetic rate constants, K1, were calculated for the reaction of these disulfides with each protein. The results show that the studied heterocyclic disulfides are the most reactive compounds with both proteins. The lipophilic properties of these disulfides were evaluated by reversed-phase high performance liquid chromatography, using the percentage of acetonitrile (PAC) required for eluting each compound of the chromatographic column in a water-acetonitrile gradient. The structure-reactivity correlations between log K1 and log PAC are stated for each protein and compared. They fit a parabolic curve which permits one to define a lipophilic domain corresponding to a quantitative reaction of disulfides towards these proteins. The studied disulfides present a similar optimum of reactivity for both proteins. |
| Databáze: | OpenAIRE |
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