The effect of iron on Trichomonas vaginalis TvCP2: a cysteine proteinase found in vaginal secretions of trichomoniasis patients
| Autor: | Beatriz Velázquez-Valassi, Esly Alejandra Euceda-Padilla, Rossana Arroyo, Jaime Ortega-López, Jesús F. T. Miranda-Ozuna, Sebastián Lorenzo-Benito, Lidia Jasso-Villazul, Bibiana Chávez-Munguía, Anel Lagunes-Guillén, Luis Alberto Rivera-Rivas, Diana Belén Sánchez-Rodríguez |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bodily Secretions Iron 030231 tropical medicine Cell Protozoan Proteins Biology medicine.disease_cause HeLa 03 medical and health sciences 0302 clinical medicine Cysteine Proteases medicine Trichomonas vaginalis Cytotoxic T cell Humans Cytotoxicity Trichomoniasis medicine.disease biology.organism_classification Molecular biology In vitro 030104 developmental biology Infectious Diseases medicine.anatomical_structure Vagina biology.protein Animal Science and Zoology Parasitology Female Antibody Research Article |
| Zdroj: | Parasitology |
| Popis: | Trichomonas vaginalis (Tv) induces host cell damage through cysteine proteinases (CPs) modulated by iron. An immunoproteomic analysis showed that trichomoniasis patient sera recognize various CPs, also some of them are present in vaginal washes (VWs). Thus, the goal of this work was to determine whether TvCP2 is expressed during infection and to assess the effect of iron on TvCP2 expression, localization and contribution to in vitro cellular damage. Western-blotting (WB) assays using TvCP2r and vaginitis patient serum samples showed that 6/9 Tv (+) but none of the Tv (−) patient sera recognized TvCP2r. WB using an anti-TvCP2r antibody and VWs from the same patients showed that in all of the Tv (+) but none of the Tv (−) VWs, the anti-TvCP2r antibody detected a 27 kDa protein band that corresponded to the mature TvCP2, which was confirmed by mass spectrometry analysis. Iron decreased the amount of TvCP2 mRNA and the protein localized on the parasite surface and cytoplasmic vesicles concomitant with the cytotoxic effect of TvCP2 on HeLa cells. Parasites pretreated with the anti-TvCP2r antibody also showed reduced levels of cytotoxicity and apoptosis induction in HeLa cell monolayers. In conclusion, these results show that TvCP2 is expressed during trichomonal infection and plays an important role in the in vitro HeLa cell cytotoxic damage under iron-restricted conditions. |
| Databáze: | OpenAIRE |
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