Antiprostate Cancer Activity of Ineupatolide Isolated from Carpesium cernuum L
Autor: | Hong-Wei Guo, Chen Yan, Lai Zhang, Jing-Xin Mao, Min Chen, Yuanshe Huang |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Article Subject Phytochemicals Apoptosis X-Linked Inhibitor of Apoptosis Protein Asteraceae Inhibitor of apoptosis General Biochemistry Genetics and Molecular Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine Humans Protein kinase B Cell Proliferation General Immunology and Microbiology medicine.diagnostic_test Chemistry Prostatic Neoplasms Cancer Cell migration General Medicine Cell cycle medicine.disease Antineoplastic Agents Phytogenic Molecular biology 030104 developmental biology 030220 oncology & carcinogenesis PC-3 Cells Cancer cell Medicine Proto-Oncogene Proteins c-akt Signal Transduction Research Article |
Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
ISSN: | 2314-6133 |
DOI: | 10.1155/2021/5515961 |
Popis: | Objective. The aim of the study was to investigate the antiprostate cancer effects and mechanism of ineupatolide (T-21), a natural product isolated from the Compositae plant Carpesium cernuum L., on PC-3 human prostate cancer cells. Methods. The effect of T-21 on the proliferation of PC-3 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell migration, and invasion experiments; the morphology of cell apoptosis was observed by Hoechst-propidium iodide staining; the effects of T-21 on PC-3 cell apoptosis and the cell cycle were evaluated by flow cytometry; and the effect of T-21 on the expression levels of phosphorylated protein kinase B (p-AKT), AKT, X-linked inhibitor of apoptosis protein (xlAP), procaspase-3, and poly (ADP-ribose) polymerase (PARP) in PC-3 cells was measured by western blotting. Results. T-21 significantly inhibited the proliferation of cells, and its half-maximal inhibitory concentrations at 12, 24, and 48 h were 38.46 ± 1.01 , 24.63 ± 0.70 , and 7.36 ± 0.58 μ M , respectively. T-21 may promote cell apoptosis in a concentration-dependent manner and block the cell cycle in the G2 and S phases. In addition, T-21 significantly reduced the protein expression levels of p-AKT, AKT, xlAP, procaspase-3, and PARP. Conclusion. T-21 exhibits antiproliferation effects on PC-3 cells by promoting apoptosis and arresting the cell cycle in the G2 and S phases. The possible mechanism underlying its potential therapeutic effects against prostate cancer is related to the AKT/xlAP pathway. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |