Exploring the role of residue 228 in substrate and inhibitor recognition by VIM Metallo-β-lactamases
| Autor: | Graciela Mahler, Christopher J. Wallace, Robert A. Bonomo, Alejandro J. Vila, Krisztina M. Papp-Wallace, Magda Kosmopoulou, Magdalena A. Taracila, Steven H. Marshall, Maria V. Villegas, Leticia I. Llarrull, James Spencer, Brigid Wilson, Maria F. Mojica, Michael E. Harris, Christopher R. Bethel |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Imipenem Klebsiella pneumoniae Stereochemistry Otras Ciencias Biológicas Microbial Sensitivity Tests medicine.disease_cause Crystallography X-Ray Biochemistry Pyrrolidine Article beta-Lactam Resistance beta-Lactamases purl.org/becyt/ford/1 [https] Ciencias Biológicas chemistry.chemical_compound Bacterial Proteins Catalytic Domain medicine polycyclic compounds Escherichia coli metallo-beta-lactamase Enzyme kinetics purl.org/becyt/ford/1.6 [https] chemistry.chemical_classification biology Chemistry Mutagenesis Substrate (chemistry) biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses inhibition Anti-Bacterial Agents Kinetics Enzyme Amino Acid Substitution VIM Pseudomonas aeruginosa Thiazolidines CIENCIAS NATURALES Y EXACTAS medicine.drug Protein Binding |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natural variant of VIM-2, possessing an R228L substitution and a novel phenotype, compelled us to explore the role of this position and its effects on substrate specificity. We employed mutagenesis, biochemical and biophysical assays, and crystallography. VIM-24 (R228L) confers enhanced resistance to cephems and increases the rate of turnover compared to that of VIM-2 (kcat/KM increased by 6- and 10-fold for ceftazidime and cefepime, respectively). Likely the R → L substitution relieves steric clashes and accommodates the C3N-methyl pyrrolidine group of cephems. Four novel bisthiazolidine (BTZ) inhibitors were next synthesized and tested against these MBLs. These inhibitors inactivated VIM-2 and VIM-24 equally well (Ki∗ values of 40-640 nM) through a two-step process in which an initial enzyme (E)-inhibitor (I) complex (EI) undergoes a conformational transition to a more stable species, E∗I. As both VIM-2 and VIM-24 were inhibited in a similar manner, the crystal structure of a VIM-2-BTZ complex was determined at 1.25 Å and revealed interactions of the inhibitor thiol with the VIM Zn center. Most importantly, BTZs also restored the activity of imipenem against Klebsiella pneumoniae and Pseudomonas aeruginosa in whole cell assays producing VIM-24 and VIM-2, respectively. Our results suggest a role for position 228 in defining the substrate specificity of VIM MBLs and show that BTZ inhibitors are not affected by the R228L substitution. Fil: Mojica, Maria F.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Mahler, S. Graciela. Universidad de la República; Uruguay Fil: Bethel, Christopher R.. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Taracila, Magdalena A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Kosmopoulou, Magda. University of Bristol; Reino Unido Fil: Papp Wallace, Krisztina M.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Wilson, Brigid M.. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Marshall, Steven H.. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Wallace, Christopher J.. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos Fil: Villegas, Maria V.. Centro Internacional de Entrenamiento e Investigaciones Medicas; Colombia Fil: Harris, Michael E.. Case Western Reserve University; Estados Unidos Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Spencer, James. University of Bristol; Reino Unido Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Veterans Affairs Medical Center; Estados Unidos |
| Databáze: | OpenAIRE |
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