p-Coumaric acid regulates macrophage polarization in myocardial ischemia/reperfusion by promoting the expression of indoleamine 2, 3-dioxygenase
| Autor: | Na Li, Rui Li, Xian-Liang Yan, Fang-Fang Yu, Xue-Yuan Guo, Jian Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Cardiotonic Agents p-coumaric acid Coumaric Acids macrophage polarization Macrophage polarization Apoptosis Myocardial Reperfusion Injury Bioengineering Inflammation CCL2 Applied Microbiology and Biotechnology In vivo medicine Animals Indoleamine-Pyrrole 2 3 -Dioxygenase Macrophage Myocytes Cardiac ARG1 Indoleamine 2 3-dioxygenase myocardial ischemia/reperfusion Chemistry Macrophages Cell Polarity General Medicine M2 Macrophage ido Mice Inbred C57BL Cancer research Cytokines Inflammation Mediators medicine.symptom TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 2, Pp 10971-10981 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | Macrophage infiltration is a hallmark pathological change observed in early stage myocardial ischemia/reperfusion (MI/R) injury and one of the main causes of myocardial damage. Here, we investigated the effects of p-Coumaric acid (p-CA) on macrophage polarization following MI/R injury and its mechanisms. In vitro, p-CA decreases the expression of LPS/IFN-γ-induced M1 macrophage markers (TNF-α, IL-6, iNOS and CCL2) and increases IL-4-induced M2 macrophage markers (IL-10, CD206, Arg1 and Mrc) in mouse bone marrow-derived macrophages (BMDMs). Additionally, p-CA elevated indoleamine 2, 3-dioxygenase (IDO) protein expression levels, M2 macrophage polarization and M2 macrophage markers through IL-4. In contrast, repression of IDO attenuated p-CA functions regulating BMDMs through IL-4. In vivo, IDO expression was downregulated in mouse hearts subjected to MI/R injury. Treatment of p-CA increased IDO expression in the hearts of MI/R mice. Functionally, p-CA decreases M1 macrophage markers, the number of M1 macrophages and inflammation around heart tissue following MI/R injury. Importantly, p-CA reduces cardiomyocyte apoptosis caused by MI/R. Altogether, our study identified that p-CA modulates macrophage polarization by promoting IDO expression and that p-CA attenuates macrophage-mediated inflammation following MI/R by promoting M2 macrophage polarization through IDO. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|