Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin
| Autor: | Ravindar R. Thomas, James P. Bennett, Francisco R. Portell, Shaharyar M. Khan, Rafal M. Smigrodzki |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Cell Survival Cellular respiration Cell Respiration Motor Activity Mitochondrion medicine.disease_cause DNA Mitochondrial Article Mitochondrial Proteins Mice chemistry.chemical_compound Adenosine Triphosphate Cell Line Tumor medicine Animals Humans Molecular Biology Neurons ATP synthase biology MPTP Cell Biology Fibroblasts TFAM Molecular biology Endotoxemia Recombinant Proteins Mitochondria Cell biology DNA-Binding Proteins Mice Inbred C57BL Oxidative Stress chemistry Mitochondrial biogenesis 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine biology.protein Molecular Medicine Adenosine triphosphate Oxidative stress Transcription Factors |
| Zdroj: | Mitochondrion. 11:108-118 |
| ISSN: | 1567-7249 |
| DOI: | 10.1016/j.mito.2010.08.004 |
| Popis: | Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP(+))-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. |
| Databáze: | OpenAIRE |
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