Comparison of the double-disk, combined disk, and Etest methods for detecting metallo-β-lactamases in gram-negative bacilli
| Autor: | Shu Huei Tsai, Chin Luan Chuang, Jiunn Jong Wu, Jing Jou Yan |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Imipenem Klebsiella pneumoniae Cefepime Ceftazidime Microbial Sensitivity Tests Biology Sensitivity and Specificity beta-Lactamases Microbiology Drug Resistance Bacterial Gram-Negative Bacteria polycyclic compounds medicine Humans Clavulanic Acid Etest Bacteriological Techniques General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Cephalosporins Citrobacter freundii Acinetobacter baumannii Infectious Diseases Culture Media Conditioned Enterobacter cloacae medicine.drug |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 49:5-11 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2004.01.002 |
| Popis: | Three phenotypic methods for detecting metallo-beta-lactamases (MBLs) in gram-negative bacilli were tested on 60 MBL producers and 155 MBL nonproducers. The 2-mercaptopropionic acid double-disk potentiation method using ceftazidime and cefepime with and without clavulanate was found to be most sensitive (100%). The combined disk method depends on comparing the zones given by disks containing a beta-lactam with and without ethylenediaminetetraacetic acid. Various drug combinations were tested, and the best results were obtained with imipenem for Pseudomonas species and Acinetobacter baumannii, ceftazidime-clavulanate for Klebsiella pneumoniae, and cefepime-clavulanate for Enterobacter cloacae and Citrobacter freundii. The overall sensitivity of the combined disk method was 86.7%. The Etest (AB BIODISK, Solna, Sweden) method using imipenem- ethylenediaminetetraacetic acid strips detected imipenem-resistant MBL-producing isolates only, resulting in a sensitivity of 36.7%. Our data suggest that the double-disk and combined disk tests are acceptable methods for MBL detection in both imipenem-resistant and imipenem-susceptible isolates. |
| Databáze: | OpenAIRE |
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