miR-29b in regulating blood pressure and cardiac function in the rat model of hypertension
| Autor: | Boya Zhang, Xiujiang Han, Yang Dong, Yongjian Li, Chao Wang, Zhe Jin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cardiac function curve Cancer Research medicine.medical_specialty hypertension viruses Virus 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) Internal medicine Medicine biology Oncogene business.industry miR-29b rat model blood pressure Articles General Medicine Transfection biology.organism_classification Molecular medicine 030104 developmental biology Endocrinology Blood pressure Apoptosis 030220 oncology & carcinogenesis Lentivirus cardiac function business |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2019.7335 |
| Popis: | The possibility of micro ribonucleic acid-29b (miR-29b) regulating blood pressure and cardiac function in the rat model of hypertension was investigated. Sixty rat models of hypertension were established and randomly divided into the lentivirus group (n=20), the negative lentivirus group (n=20) and the control group (n=20). Rats in the lentivirus group were injected with the recombinant lentivirus, and those in the negative lentivirus and control groups were injected with the negative control virus and infection enhancement solution, respectively. The systolic pressure of rats was monitored using the tail-cuff method, and changes in the cardiac function of rats were evaluated via high-frequency ultrasound. At 3 weeks after virus infection, rats were weighed and sacrificed, the heart was taken and the left ventricular mass index was calculated. Moreover, the expression of miR-29b in myocardial tissues was detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The systolic pressure in the lentivirus group was significantly decreased compared with those in the negative lentivirus and control groups (P |
| Databáze: | OpenAIRE |
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