Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano

Autor:	Wudarski, Jakub, Ustyantsev, Kirill, Reinoite, Filipa, Berezikov, Eugene, Blanchoud, Simon, Galliot, Brigitte
Přispěvatelé:	Stem Cell Aging Leukemia and Lymphoma (SALL), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Animals Genetically Modified Platyhelminths Macrostomum lignano Stem Cells Flatworms Gene Transfer Techniques Animals Regeneration Irradiation Transgenesis Random integration Microinjection Research Article
Zdroj:	Whole-Body Regeneration: Methods and Protocols, 493-508 STARTPAGE=493;ENDPAGE=508;TITLE=Whole-Body Regeneration Methods in Molecular Biology ISBN: 9781071621714 Methods Mol Biol
Popis:	Regeneration-capable flatworms are highly informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. Transgenesis is a powerful research tool for investigating gene function, but until recently, a transgenesis method was missing in flatworms, hampering their wider adoption in biomedical research. Here we describe a detailed protocol to create stable transgenic lines of the flatworm M. lignano using random integration of DNA constructs through microinjection into single-cell stage embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c5c29440325255edb8aebe80bc46c54 https://research.rug.nl/en/publications/de985d87-a4b3-4d2f-906b-fd3f839eef75 Zobrazit plný text záznamu