Differences in hydrolytic abilities of two crude lipases from Geotrichum candidum 4013
| Autor: | Martina Mackova, Marie Zarevúcka, Jana Brabcová |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Triacylglycerol lipase
Bioengineering Applied Microbiology and Biotechnology Biochemistry Michaelis–Menten kinetics Substrate Specificity Fungal Proteins chemistry.chemical_compound Hydrolysis Stearate Genetics Extracellular Lipase Triglycerides chemistry.chemical_classification Fungal protein Chromatography biology Fatty acid Geotrichum Kinetics chemistry biology.protein Biotechnology |
| Zdroj: | Yeast. 27:1029-1038 |
| ISSN: | 0749-503X |
| DOI: | 10.1002/yea.1812 |
| Popis: | The fungus Geotrichum candidum 4013 produces two types of lipases (extracellular and cell-bound). Both enzymes were tested for their hydrolytic ability to p-nitrophenyl esters and compounds having a structure similar to the original substrate (triacylglycerols). Higher lipolytic activity of extracellular lipase was observed when triacylglycerols of medium- (C12) and long- (C18) chain fatty acids were used as substrates. Cell-bound lipase preferentially hydrolysed trimyristate (C14). The differences in the abilities of these two enzymes to hydrolyse p-nitrophenyl esters were observed as well. The order of extracellular lipase hydrolysis relation velocity was as follows: p-nitrophenyl decanoate > p-nitrophenyl caprylate > p-nitrophenyl laurate > p-nitrophenyl palmitate > p-nitrophenyl stearate. The cell-bound lipase indicates preference for p-nitrophenyl palmitate. The most striking differences in the ratios between the activity of both lipases (extracellular : cell-bound) towards different fatty acid methyl esters were 2.2 towards methyl hexanoate and 0.46 towards methyl stearate (C18). The Michaelis constant (K(m) ) and maximum reaction rate (V(max) ) for p-nitrophenyl palmitate hydrolysis of cell-bound lipase were significantly higher (K(m) 2.462 mM and V(max) 0.210 U/g/min) than those of extracellular lipase (K(m) 0.406 mM and V(max) 0.006 U/g/min). |
| Databáze: | OpenAIRE |
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