Intermediate- and low-molecular-weight keratin detection with the monoclonal antibody MNF116. An immunohistochemical study on 232 paraffin-embedded cutaneous lesions
| Autor: | J Lugo, Victor G. Prieto, N. S. McNutt |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Skin Neoplasms Histology medicine.drug_class Dermatology Biology Monoclonal antibody Skin Diseases Stain Pathology and Forensic Medicine Cytokeratin Basal (phylogenetics) Keratin medicine Humans Prospective Studies Melanoma Retrospective Studies chemistry.chemical_classification Paraffin Embedding Antibodies Monoclonal Immunohistochemistry Staining Molecular Weight medicine.anatomical_structure chemistry Carcinoma Basal Cell Carcinoma Squamous Cell Keratins Merkel cell |
| Zdroj: | Journal of Cutaneous Pathology. 23:234-241 |
| ISSN: | 1600-0560 0303-6987 |
| DOI: | 10.1111/j.1600-0560.1996.tb01472.x |
| Popis: | Immunohistochemical detection of certain low to intermediate molecular weight keratins often is impaired in routinely processed specimens due to masking of these antigens by formalin fixation. Despite standard enzymatic digestion, AE1:AE3 and CAM 5.2, two of the most currently utilized antikeratin antibody preparations, either stain weakly or fail to stain basal keratinocytes and tumors composed of basaloid keratinocytes in paraffin sections of formalin-fixed tissue. We present here our experience with the monoclonal antibody MNF116 which detects keratins 5, 6, 8, 17, and 19 (DAKO, Carpinteria, CA). We have studied 232 routinely-processed skin lesions with MNF116 and compared the staining with that of AE1:AE3 mixture or CAM 5.2. In normal skin, the staining achieved with MNF116 was particularly strong on the basal cells of the epidermis and adnexae. MNF116 was positive in all 154 epithelial tumors and negative in all but one (a leiomyosarcoma) of 78 mesenchymal and melanocytic tumors. AE1:AE3 mixture was positive in all but four poorly-differentiated squamous cell carcinomas and it was only weakly positive in most basal cell carcinomas. CAM 5.2 was positive in tumors of the sweat apparatus, Merkel cell carcinomas, metastatic carcinomas, and 5/15 basal cell carcinomas. We consider that, in routinely processed specimens, MNF116 is very useful and convenient for detection of cytokeratin expression in cutaneous lesions, and therefore helpful in the evaluation of tumors with small cells and other poorly differentiated neoplasms of the skin. |
| Databáze: | OpenAIRE |
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