Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai
| Autor: | M. Afzal Ghauri, Onur Kırtel, Wim Van den Ende, Stephen R. Lindemann, Anam Nasir, Fazal Sattar, Ming-Hsu Chen, Munir A. Anwar, Iram Ashfaq, Shazia Khaliq, Ebru Toksoy Ӧner |
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| Přispěvatelé: | Nasir, Anam, Sattar, Fazal, Ashfaq, Iram, Lindemann, Stephen R., Chen, Ming-Hsu, Van den Ende, Wim, Oner, Ebru Toksoy, Kirtel, Onur, Khaliq, Shazia, Ghauri, M. Afzal, Anwar, Munir A. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
STRUCTURAL-CHARACTERIZATION Bacillus aryabhattai STRAIN Sucrose 01 natural sciences Levan chemistry.chemical_compound 0404 agricultural biotechnology 13c nmr spectroscopy Biosynthesis Exopolysaccharide 010608 biotechnology Fructooligosaccharides Food science OPTIMIZATION Rhizosphere LEVANSUCRASE Chemistry 04 agricultural and veterinary sciences 040401 food science Thin-layer chromatography Prebiotics FRUCTANSUCRASE ENZYMES Molar mass distribution Aeration Food Science |
| Popis: | Bacillus aryabhattai GYC2-3 strain, capable of synthesizing EPS, was isolated from the rhizosphere of Taraxacum spp. plant. The EPS was identified as levan by C-13 NMR spectroscopy and methylation analysis. The levan produced had a high weight average molecular weight of 5.317 x 10(7) Da and degree of branching 5.19%. Thin layer chromatography and high-performance anion-exchange chromatography revealed that it is capable of producing broad range of fructooligosaccharides (FOS) and higher levels of FOS synthesis were observed with increasing sucrose concentrations. The optimum temperature, initial pH and sucrose concentration for levan biosynthesis were studied to be 30 degrees C, 8.0 and 250 g/L, respectively. Levan production was increased by similar to 38% (26 g/L) when provided aeration as compare to static cultures. To the best of our knowledge, this is the first report on biosynthesis of levan and FOS by a B. aryabhattai strain. |
| Databáze: | OpenAIRE |
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