Clinical potential role of circulating microRNAs in early diagnosis of colorectal cancer patients
| Autor: | Haiyan Chu, Lingjun Zhu, Zhengdong Zhang, Meilin Wang, Meiyun Kang, Danni Shi, Dongying Gu, Qiaoyan Wang, Na Tong, Tamara S. Adams, Jinfei Chen, Sang Liu, Mulong Du |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology Cancer Research medicine.medical_specialty Colorectal cancer Bioinformatics Meta-Analysis as Topic Internal medicine microRNA Biomarkers Tumor medicine Humans Aged Aged 80 and over Plasma samples Reverse Transcriptase Polymerase Chain Reaction business.industry Curve analysis Healthy subjects Case-control study General Medicine Middle Aged Prognosis medicine.disease digestive system diseases MicroRNAs Circulating MicroRNA Early Diagnosis ROC Curve Area Under Curve Case-Control Studies Biomarker (medicine) Female Neoplasm Grading Colorectal Neoplasms business |
| Zdroj: | Carcinogenesis. 35:2723-2730 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/bgu189 |
| Popis: | Current procedures for diagnosis and biomarker examination of colorectal cancer (CRC) are invasive and unpleasant. There is a great need to identify sensitive and specific biomarkers for early diagnosis of CRC. Circulating microRNAs (miRNAs) are promising molecular markers for CRC prediction. We performed a comprehensive meta-analysis to integrate an evaluation index for diagnostic accuracy of circulating miRNAs in diagnosing CRC patients. Furthermore, we conducted an independent validation set of 49 CRC patients and 49 healthy controls. In our meta-analysis, we found that miR-21 yielded a pooled area under ROC curve (AUC) of 0.867 (sensitivity: 76%, specificity: 82%) in discriminating CRC from controls, and miR-92a yielded a summary AUC of 0.803 (sensitivity: 77%, specificity: 68%); miR-21 had a higher diagnostic efficiency than miR-92a. In the further validation, plasma miR-21 levels in CRC patients were significantly higher than levels observed in healthy subjects. A ROC curve analysis showed a consistent result. However, this phenotype was not present in miR-92a. Moreover, the expression trend of miR-21 in plasma samples was in line with that of tissue samples, along with the cellular level. Current evidences suggest that plasma miR-21 could be a reliable and non-invasive biomarker for CRC diagnosis. Studies with larger cohorts that include the diagnostic value of plasma miR-21 for CRC are warranted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|