Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity

Autor: G. Del Giudice, Claudio Donati, Domenico Maione, Giulia Torricelli, Flora Castellino, C. Brettoni, V. Masignani, Silvia Guidotti, Erica Borgogni, Alessandro Muzzi, M. Martinelli, S. Iozzi, Roberto Petracca, Marco Bruttini, Erika Bartolini, Rino Rappuoli, Mariagrazia Pizza, Marzia Monica Giuliani, Franco Felici, Sara Marchi, Concetta Beninati, Maria Domina, S. Bonacci, Giuseppe Teti
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Adult
0301 basic medicine
Protein vaccines
Adolescent
Science
Protein Array Analysis
General Physics and Astronomy
Meningococcal Vaccines
Neisseria meningitidis
Serogroup B
Serum Bactericidal Antibody Assay
4CMenB vaccine
medicine.disease_cause
Article
Antibodies
General Biochemistry
Genetics and Molecular Biology
Young Adult
03 medical and health sciences
0302 clinical medicine
Antibody Repertoire
Antigen
Peptide Library
medicine
Humans
Child
lcsh:Science
Antigens
Bacterial
Settore BIO/11 - BIOLOGIA MOLECOLARE
Multidisciplinary
biology
Neisseria meningitidis
Infant
General Chemistry
Antibodies
Bacterial
Meningococcal Infections
Vaccination
030104 developmental biology
Epitope mapping
Child
Preschool
Immunology
Protein microarray
biology.protein
lcsh:Q
Bacterial infection
Antibody
Epitope Mapping
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Nature Communications
Popis: Serogroup B meningococcus (MenB) is a leading cause of meningitis and sepsis across the world and vaccination is the most effective way to protect against this disease. 4CMenB is a multi-component vaccine against MenB, which is now licensed for use in subjects >2 months of age in several countries. In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the three major 4CMenB antigenic components (fHbp, NHBA and NadA) in individual sera from vaccinated infants, adolescents and adults. The resulting 4CMenB protein antigen fingerprinting allowed the identification of specific human antibody repertoire correlating with the bactericidal response elicited in each subject. This work represents an example of epitope mapping of the immune response induced by a multicomponent vaccine in different age groups with the identification of protective signatures. It shows the high flexibility of this microarray based methodology in terms of high-throughput information and minimal volume of biological samples needed.
4CMenB is an approved multi-component vaccine against Serogroup B meningococcus. Here the authors develop a protein microarray for three major 4CMenB antigenic components (fHbp, NHBA and NadA) and describe antibody repertoires in sera from vaccinated infants, adolescents and adults correlating with bactericidal response.
Databáze: OpenAIRE
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