Tuberculose extrapulmonar em pacientes com AIDS na cidade do Rio Grande, Brasil. Diagnóstico laboratorial
Autor: | Maria Marta Santos Boffo, Marta O. Ribeiro, Suzana Jardim, Ivo Gomes de Mattos |
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Rok vydání: | 2000 |
Předmět: |
lcsh:RC705-779
Extrapulmonary tuberculosis / AIDS Pulmonary and Respiratory Medicine Aids patients Pathology medicine.medical_specialty Tuberculosis biology business.industry Extrapulmonary tuberculosis lcsh:Diseases of the respiratory system University hospital medicine.disease biology.organism_classification Stain Sodium salt Mycobacterium tuberculosis medicine.anatomical_structure medicine Tuberculose/diagnóstico laboratorial tuberculosis / laboratory diagnosis Tuberculose extrapulmonar/AIDS business Lymph node |
Zdroj: | Revista Portuguesa de Pneumologia, Vol 6, Iss 4, Pp 277-282 (2000) |
ISSN: | 0873-2159 |
DOI: | 10.1016/s0873-2159(15)30906-5 |
Popis: | RESUMO: Foram analisadas 102 amostras clÃnicas de sÃtios extrapulmonares refereotes a 74 pacieotes com AIDS, entre Setembro de 1997 e Dezembro de 1999, do Serviço de AIDS do Hospital Universitário da Universidade Federal de Rio Grande, na cidade de Rio Grande, RS, Brasil. Este estudo teve como objetivo enfatizar a importância do diagnóstico laboratorial da tuberculose extrapulmonar nos padentes com AIDS e com suspelta clÃnica dessa enfermidade. Foi realizada baciloscopla a partir de esfregaços corados pela técnica de Kinyoun e cultivo primário para isolamento em melo de Ogawa e Ogawa acresido de ácido p-nitrobenzóico, de ácido tiofeno-2-carboxÃlico e de piruvato de sódio. Foi feita a determinação da produção de niacina para confirmação do isolamento de Mycobacterium tuberculosis. O diagnóstico de tuberculose extrapulmonar foi obtido em 12,74% das amostras provenientes de 17% dos pacientes. A distribuição das amostras com resultados positivos quanto ao sÃtio de obtenção, inclui: 8/13 blópsias ganglionares, 2/13 biópsias hepáticas, 2/13 lÃquido ascÃtico e 1/13 lÃquido pelural. A baciloscopla foi positiva em 7/13 amostras e a cultura em 10/13. Em 3/13 casos a baciloscopia foi a única responsável pelo diagnóstico de tuberculose extrapulmonar e a cultura em 6/13. Das amostras positivas à baciloscopia 6/7 eram biópsias ganglionares e 1/7, lÃquido ascÃtico. Os dois métodos utilizados na busca do diagnóstico da forma extrapulmonar de tuberculose, mostraram um rendimento que nos leva a incentivar cada vez mais a realização sistemática dos mesmos em nosso meio.REV PORT PNEUMOL 2000; VI (4): 277-282 ABSTRACT: One hundred two specimens of extrapulmonary material from 74 AIDS patients from the AIDS Service of University Hospital, FURG, Rio Grande, RS, Brazil, were examined at Mycobacteria Laboratory, between September 1997 and December 1999. This study had the aim to evaluate laboratoy importance in the diagnosis of extrapulmonary tuberculosis in AIDS patients with clinical suggestive symptoms of tuberculosis. Acid-fast bacilli (AFB) stains were performed using the Kinyon Method (cold stainning). Mycobacterial isolation was made In the Ogawa medium. The Ogawa medium added with p-nitrobenzoic acid, 2-tiophenocarboxylic acid hidrazide and pyruvic acid sodium salt was used to the Identification of mycobacterial species. Confirmation of Mycobacterium tuberculosis was done by the niacin test. The extrapulmonary tuberculosis diagnosis was done in 12,74% of specimens from 17,56% of the patients. Distribution of specimens with positive results in relation to the obtained sites were: 8/13 lymph node biopsies, 2/13 liver biopsies, 2/13 ascitic nuid and 1/13 pleural nuid. ABF were found on microscopic examination from 7/13 specimens and 10/13 in culture. The microscopic examination of ABF stain was the only method responsible for the extrapulmonary tuberculosis diagnostic in 3/13 cases and the culture in 6/13 cases. Among the positive specimens at microscopic examination or ABF stains, 6/7 were lymph node biopsies and In ascitic fluid. The used methods in the diagnosis, of the extrapulmonary tuberculosis showed good results, by this way we strongly recommend the realization of both as routine in our place.REV PORT PNEUMOL 2000; VI (4): 277-282 Palavras-chave: Tuberculose/diagnóstico laboratorial, Tuberculose extrapulmonar/AIDS, Key-words: tuberculosis,/ laboratory diagnosis, Extrapulmonary tuberculosis / AIDS |
Databáze: | OpenAIRE |
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