Quantitation of the ABO System Antigens by Means of Isoprecipitating Antisera
| Autor: | Zelená E, Pintera J, Vacl J, Hrncírová D, Kurtinová J |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Antiserum Counterimmunoelectrophoresis education.field_of_study Saliva Chromatography biology Chemistry Population Hematology Standard solution ABO Blood-Group System Precipitins Antigen Isoantibodies ABO blood group system Immunology biology.protein Chemical Precipitation Humans Female Titration Antibody education |
| Zdroj: | British Journal of Haematology. 30:75-80 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1975.tb00520.x |
| Popis: | A quantitative method for the determination of blood group specific substances A and B on red blood cells was developed, using them to absorb isoprecipitating antisera anti-A and anti-B, followed by estimation of the residual antibody by means of a counterimmunoelectrophoretic technique with standard solutions of A and B substances prepared from saliva. The method was tested on a population of A2B donors. The mean amount of A substance in A2B RBC was 131 X 10(-12) +/- 217 X 10(-12) u/cell in the population of 95 donors. The A substance thus determined agreed significantly with values obtained by classical haemagglutinating titration. The mean amount of B substance in A2B RBC was 222 X 10(-12) +/- 178 X 10(-12) m/cell. The amounts of both the A and B substances were not correlated on the same RBC. Several peaks of the distribution curve of both substances confirmed the non-homogeneity of the populations, there being a wide standard deviation presumably due to differences in the antigen content of different subgroups. |
| Databáze: | OpenAIRE |
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