Cutaneous Thermal Injury Modulates Blood and Skin Metabolomes Differently in a Murine Model
| Autor: | Gaurav Garg, Jeffrey W. Shupp, Raina Kumar, Abdulnaser Alkhalil, Robert L. Ball, Lauren T. Moffatt, A Day, Bonnie C. Carney, Rasha Hammamieh |
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| Rok vydání: | 2020 |
| Předmět: |
Burn injury
Hot Temperature AcademicSubjects/MED00910 Pharmacology Pentose phosphate pathway Mice 03 medical and health sciences 0302 clinical medicine Metabolomics Metabolome Animals Medicine 030304 developmental biology 0303 health sciences Thermal injury business.industry Rehabilitation Original Articles Metabolism Pathophysiology Disease Models Animal 030220 oncology & carcinogenesis Emergency Medicine Surgery Burns business Total body surface area Biomarkers |
| Zdroj: | Journal of Burn Care & Research: Official Publication of the American Burn Association |
| ISSN: | 1559-0488 1559-047X |
| DOI: | 10.1093/jbcr/iraa209 |
| Popis: | As the field of metabolomics develops further, investigations of how the metabolome is affected following thermal injury may be helpful to inform diagnostics and guide treatments. In this study, changes to the metabolome were tested and validated in a murine burn injury model. After a 30% total body surface scald injury or sham procedure sera and skin biopsies were collected at 1, 2, 6, or 24 hr. Burn-specific changes in the metabolome were detected compared to sham animals. The sera metabolome exhibited a more rapid response to burn injury than that of the skin and it peaked more proximal to injury (6 vs 24 hr). Progression of metabolic response in the skin was less synchronous and showed a higher overlap of the significantly modified metabolites (SMMs) among tested time-points. Top affected pathways identified by SMMs of skin included inositol phosphate metabolism, ascorbate and alderate metabolism, caffeine metabolism, and the pentose phosphate pathway. Future research is warranted in human and larger animal models to further elucidate the role of metabolomic perturbations and the pathophysiology following burn injury. |
| Databáze: | OpenAIRE |
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