Preparation and evaluation of antigens used in serological tests fo caprine syncytial retrovirus antibody in sheep and goat sera
| Autor: | V.W. Smith, W. Coackley, D.J. Houwers |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Immunodiffusion
Sheep Visna-maedi virus General Veterinary biology Visna virus Goats Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena General Medicine Antibodies Viral biology.organism_classification Microbiology Virology Virus Serology Retroviridae Virus antigen Antigen biology.protein Animals Antibody Antigens Viral |
| Zdroj: | Veterinary Microbiology. 9:581-586 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/0378-1135(84)90020-8 |
| Popis: | Methods used to prepare antigens from caprine syncytial retrovirus (CSR) for use in the agarose gel immunodiffusion test (AGID) or an indirect enzyme-linked immunosorbent assay (ELISA) are described. Caprine and ovine sera were tested for antibody to CSR using the AGID test and ELISA incorporating a caprine system (CSR antigen and rabbit anti-goat IgG) or an ovine system (maedi-visna virus antigen and rabbit anti-sheep IgG). Good correlation was achieved in the results of the 3 tests when sera were devoid of antibody or were strongly positive. Variations in the results on weakly positive sera were considered to be more a matter of interpretation than due to basic differences in the reagents employed. |
| Databáze: | OpenAIRE |
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