Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles

Autor: Hee-Kyoung Kim, Da-Woon Kim, Christian M. K. Sieber, Stefano Tonti, Hans-Ulrich Humpf, Martin Münsterkötter, Slavica Janevska, Eva-Maria Niehaus, Il-Pyung Ahn, Sung-Hwan Yun, Petra M. Houterman, Ulrich Güldener, Birgit Arndt, Bettina Tudzynski, Svetlana A. Kalinina, I. Alberti
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Gene Expression
Artificial Gene Amplification and Extension
Plant Science
Pathology and Laboratory Medicine
Biochemistry
Polymerase Chain Reaction
chemistry.chemical_compound
Fusarium
Gene cluster
Medicine and Health Sciences
Plant Hormones
Biology (General)
Phylogeny
Fungal Pathogens
Genetics
Virulence
biology
Plant Biochemistry
Fungal genetics
Eukaryota
food and beverages
Genomics
Plants
Experimental Organism Systems
Medical Microbiology
Bakanae
Pathogens
Fusaric acid
Research Article
medicine.drug
QH301-705.5
Genes
Fungal
Immunology
Mycology
Secondary metabolite
Research and Analysis Methods
Microbiology
Fungal Proteins
03 medical and health sciences
Plant and Algal Models
Virology
medicine
Grasses
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Gene
Plant Diseases
Organisms
Biology and Life Sciences
Computational Biology
Oryza
Comparative Genomics
RC581-607
biology.organism_classification
Hormones
Gibberellins
Beauvericin
030104 developmental biology
chemistry
Seedlings
Fusariosis
Parasitology
Rice
Immunologic diseases. Allergy
Zdroj: PLoS Pathog. 13:e1006670 (2017)
PLoS Pathogens, Vol 13, Iss 10, p e1006670 (2017)
PLoS Pathogens
Popis: Fusarium fujikuroi causes bakanae (“foolish seedling”) disease of rice which is characterized by hyper-elongation of seedlings resulting from production of gibberellic acids (GAs) by the fungus. This plant pathogen is also known for production of harmful mycotoxins, such as fusarins, fusaric acid, apicidin F and beauvericin. Recently, we generated the first de novo genome sequence of F. fujikuroi strain IMI 58289 combined with extensive transcriptional, epigenetic, proteomic and chemical product analyses. GA production was shown to provide a selective advantage during infection of the preferred host plant rice. Here, we provide genome sequences of eight additional F. fujikuroi isolates from distant geographic regions. The isolates differ in the size of chromosomes, most likely due to variability of subtelomeric regions, the type of asexual spores (microconidia and/or macroconidia), and the number and expression of secondary metabolite gene clusters. Whilst most of the isolates caused the typical bakanae symptoms, one isolate, B14, caused stunting and early withering of infected seedlings. In contrast to the other isolates, B14 produced no GAs but high amounts of fumonisins during infection on rice. Furthermore, it differed from the other isolates by the presence of three additional polyketide synthase (PKS) genes (PKS40, PKS43, PKS51) and the absence of the F. fujikuroi-specific apicidin F (NRPS31) gene cluster. Analysis of additional field isolates confirmed the strong correlation between the pathotype (bakanae or stunting/withering), and the ability to produce either GAs or fumonisins. Deletion of the fumonisin and fusaric acid-specific PKS genes in B14 reduced the stunting/withering symptoms, whereas deletion of the PKS51 gene resulted in elevated symptom development. Phylogenetic analyses revealed two subclades of F. fujikuroi strains according to their pathotype and secondary metabolite profiles.
Author summary Fusarium fujikuroi causes bakanae disease of rice. Infected seedlings appear to be taller and more slender when compared to healthy seedlings due to its ability to produce gibberellic acids (GAs). The disease is responsible for high yield losses, and its incidence varies with regions, rice cultivars grown and the aggressiveness of the fungal isolates. However, not all infected seedlings show bakanae symptoms: one of the isolates, B14, causes stunting and early withering of infected seedlings. The reason for the two pathotypes is not well understood. Researchers thought that the stunting phenotype was mostly caused by fungal-derived secondary metabolites such as fusaric acid, but there is no experimental evidence yet. B14 differs from the other strains by the presence of more PKS gene clusters, low expression of GA genes, lack of detectable levels of GAs and the production of high amounts of fumonisins in rice. Analysis of additional field isolates revealed a strong correlation between the pathotype (bakanae or stunting) and either GA or fumonisin production. Based on phylogenetic analyses, F. fujikuroi strains can be divided into two phylogenetically distinct subclades according to their pathotype and secondary metabolite profiles. This study provides new insights into the genomic variations and the population structure inside the species F. fujikuroi which will help to develop disease control strategies for this rice pathogen.
Databáze: OpenAIRE
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