Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi
| Autor: | Kajitani, T., Kato, H., Chikashige, Y., Tsutsumi, C., Hiraoka, Y., Kimura, Hiroshi, Ohkawa, Y., Obuse, C., Hermand, D., Murakami, Y. |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Heterochromatin NcRNA transcription Cell Cycle Proteins RNA polymerase II RNA Polymerase II/genetics Noncoding RNA noncoding RNA Chromodomain 03 medical and health sciences Long Noncoding/genetics Protein Domains RNA interference Schizosaccharomyces/genetics Schizosaccharomyces Serine Gene silencing RNA Long Noncoding/genetics RNA Small Interfering RNA Small Interfering/genetics Cell Cycle Proteins/genetics Serine/genetics Fungal/genetics Multidisciplinary biology Chemistry fungi heterochromatin RNA Fungal Heterochromatin/genetics Non-coding RNA Chromatin Cell biology RNA Fungal/genetics 030104 developmental biology PNAS Plus Small Interfering/genetics RNAi biology.protein RNA Schizosaccharomyces pombe Proteins/genetics RNA Long Noncoding Schizosaccharomyces pombe Proteins |
| Zdroj: | Kajitani, T, Kato, H, Chikashige, Y, Tsutsumi, C, Hiraoka, Y, Kimura, H, Ohkawa, Y, Obuse, C, Hermand, D & Murakami, Y 2017, ' Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi ', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 52, pp. E11208-E11217 . https://doi.org/10.1073/pnas.1714579115 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Some long noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (RNAPII) are retained on chromatin, where they regulate RNAi and chromatin structure. The molecular basis of this retention remains unknown. We show that in fission yeast serine 7 (Ser7) of the C-terminal domain (CTD) of RNAPII is required for efficient siRNA generation for RNAi-dependent heterochromatin formation. Surprisingly, Ser7 facilitates chromatin retention of nascent heterochromatic RNAs (hRNAs). Chromatin retention of hRNAs and siRNA generation requires both Ser7 and an RNA-binding activity of the chromodomain of Chp1, a subunit of the RNA-induced transcriptional silencing (RITS) complex. Furthermore, RITS associates with RNAPII in a Ser7-dependent manner. We propose that Ser7 promotes cotranscriptional chromatin retention of hRNA by recruiting the RNA-chromatin connector protein Chp1, which facilitates RNAi-dependent heterochromatin formation. Our findings reveal a function of the CTD code: linking ncRNA transcription to RNAi for heterochromatin formation. |
| Databáze: | OpenAIRE |
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