Primary effusion lymphoma cell lines harbouring human herpesvirus type-8
| Autor: | Daniela Bontempo, Gianluca Gaidano, Antonino Carbone, Tiziana Perin, Rachele Volpe, Vincenzo Canzonieri, Anna Maria Cilia, Annunziata Gloghini, Umberto Tirelli, Daniela Capello |
|---|---|
| Přispěvatelé: | Carbone, A, Cilia, Am, Gloghini, A, Capello, D, Perin, T, Bontempo, D, Canzonieri, V, Tirelli, U, Volpe, R, Gaidano, G |
| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Lymphoma viruses virus diseases Hematology biochemical phenomena metabolism and nutrition Biology medicine.disease Serous fluid Oncology immune system diseases Cell culture hemic and lymphatic diseases Herpesvirus 8 Human medicine Tumor Cells Cultured Humans Primary effusion lymphoma Human herpesvirus |
| Zdroj: | Scopus-Elsevier Università degli Studi del Piemonte Orientale-IRIS |
| Popis: | Primary effusion lymphoma (PEL) is a novel lymphoma entity consistently infected by HHV-8 that occurs predominantly in immunodeficient patients and is characterized by liquid growth in the serous body cavities. in order to facilitate the understanding of PEL pathogenesis and histogenesis, we have established three PEL cell lines termed CRO-AP/2, CRO-AP/3 and CRO-AP/5. All cell lines have been derived from HIV positive homosexual men affected by PEL with tin the case of CRO-AP/2 and CRO-AP/5) or without tin the case of CRO-AP/3) a previous history of Kaposi's sarcoma. The cell lines are representative of both virologic variants of PEL, i.e. HHV-8(+) EBV+ PEL (CRO-AP/2 and CRO-AP/5) and HHV-8(+) EBV- PEL (CRO-AP/3). Morphologic and phenotypic features of CRO-APR, CRO-AP/3 and CRO-AP/5 are typical of PEL, and include morphology bridging immunoblastic and anaplastic features as well as an indeterminate (non B- non T-cell) phenotype. The B-cell nature of the cell lines is documented by the presence of rearranged immunoglobulin genes. The detailed analysis of the molecular and phenotypic features of CRO-AP/2, CRO-AP/3 and CRO-AP/5 has allowed the identification of recurrent chromosomal abnormalities of PEL and has contributed to the definition of PEL as a lymphoma of post-germinal center, pre-terminally differentiated B-cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|