Nucleologenesis inTrypanosoma cruzi
| Autor: | María de Lourdes Segura-Valdez, Reyna Lara-Martínez, Roberto Hernández, Luis Felipe Jiménez-García, Tomás Nepomuceno-Mejía |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cell Nucleus
0301 basic medicine Nucleologenesis Nucleoplasm 030102 biochemistry & molecular biology Cell division Nucleolus Chemistry Trypanosoma cruzi Mitosis Nucleolar Organizer Region Cell biology 03 medical and health sciences 030104 developmental biology RNA Ribosomal Animals Nucleolus organizer region Instrumentation Cell Nucleolus Ribonucleoprotein |
| Zdroj: | Microscopy and Microanalysis. 22:621-629 |
| ISSN: | 1435-8115 1431-9276 |
| Popis: | Nucleolar assembly is a cellular event that requires the synthesis and processing of ribosomal RNA, in addition to the participation of pre-nucleolar bodies (PNBs) at the end of mitosis. In mammals and plants, nucleolar biogenesis has been described in detail, but in unicellular eukaryotes it is a poorly understood process. In this study, we used light and electron microscopy cytochemical techniques to investigate the distribution of nucleolar components in the pathway of nucleolus rebuilding during closed cell division in epimastigotes ofTrypanosoma cruzi, the etiologic agent of American trypanosomiasis. Silver impregnation specific for nucleolar organizer regions and an ethylenediaminetetraacetic acid regressive procedure to preferentially stain ribonucleoprotein revealed the conservation and dispersion of nucleolar material throughout the nucleoplasm during cell division. Furthermore, at the end of mitosis, the argyrophilic proteins were concentrated in the nucleolar organizer region. Unexpectedly, accumulation of nucleolar material in the form of PNBs was not visualized. We suggest that formation of the nucleolus in epimastigotes ofT. cruzioccurs by a process that does not require the concentration of nucleolar material within intermediate nuclear bodies such as mammalian and plant PNBs. |
| Databáze: | OpenAIRE |
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