DETECTION OFMYCOBACTERIUM AVIUMSUBSPECIESPARATUBERCULOSISBY IS900-BASED PCR ASSAYS FROM AN ALPACA (VICUGNA PACOS) KEPT IN A GERMAN ZOOLOGICAL GARDEN
| Autor: | Claus-Peter Czerny, Inger Völkel, Aloys von Buchholz, Pia Münster |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Veterinary medicine 040301 veterinary sciences Paratuberculosis Vicugna pacos Polymerase Chain Reaction law.invention 0403 veterinary science 03 medical and health sciences law Germany biology.domesticated_animal medicine Animals Polymerase chain reaction Feces 030304 developmental biology 0303 health sciences General Veterinary biology 04 agricultural and veterinary sciences General Medicine Amplicon medicine.disease biology.organism_classification Mycobacterium avium subspecies paratuberculosis Virology 3. Good health Mycobacterium avium subsp. paratuberculosis GenBank Animals Zoo Animal Science and Zoology Camelids New World Camelid |
| Zdroj: | Journal of Zoo and Wildlife Medicine. 44:176-180 |
| ISSN: | 1937-2825 1042-7260 |
| DOI: | 10.1638/1042-7260-44.1.176 |
| Popis: | An alpaca (Vicugna pacos) bred and kept in a German zoological garden exhibited clinical signs consistent with paratuberculosis. The presence of Mycobacterium avium ssp. paratuberculosis (MAP) was confirmed in feces and in the ileocecal lymph node (ILN) by IS900-based polymerase chain reaction (PCR) assays and culture. A bacterial burden of 7.6 x 10(6) MAP/g in feces and 4.4 x 10(7) MAP/g in lymph node tissue was determined by real-time PCR. For further characterization, a conventional PCR was developed. After sequencing of the 864-bp PCR amplicon covering nucleotide positions 13 to 876 within the IS900, the alpaca isolate shared 100% nucleotide homology with the bovine MAP-K10 IS900 reference sequence (GenBank: AE16958), indicating a cattle strain. This report supports the present occurrence of MAP in German camelid populations and highlights the need to expand routine MAP surveillance to South American camelids held in European zoos. |
| Databáze: | OpenAIRE |
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