New axonemal dynein heavy chains from Tetrahymena thermophila
| Autor: | Xuejun Kong, Jeanell D. Sullivan, Paula S. Mobberley, David G. Pennock, Steven P. Angus |
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| Rok vydání: | 1999 |
| Předmět: |
Axoneme
Nexin biology Cilium Dynein Blotting Western Tetrahymena Dyneins macromolecular substances biology.organism_classification Microbiology Tetrahymena thermophila Biochemistry Dynein ATPase Biophysics biology.protein Dynactin Centrifugation Density Gradient Animals Vanadate Electrophoresis Polyacrylamide Gel Cilia |
| Zdroj: | The Journal of eukaryotic microbiology. 46(2) |
| ISSN: | 1066-5234 |
| Popis: | Two dyneins can be extracted from Tetrahymena ciliary axonemes. The 22S dynein contains three heavy chains (HC), sediments at 22S in a sucrose gradient, and makes up the outer arms. The 14S dynein contains two to six HCs, sediments at 14S, and is thought to contribute to formation of the inner arms. We have identified two large proteins that are extracted from Tetrahymena axonemes with high salt and that sediment together at approximately 18S. The two large proteins cleave when subjected to UV light in the presence of ATP and vanadate, suggesting both proteins are dynein HC. Antibodies against one of the 18S HCs do not recognize 22S dynein HCs. Antibodies to 22S dynein HC do not bind appreciably to 18S dynein photocleavage fragments. Taken together, these results indicate that the large proteins that sediment at 18S are axonemal dynein heavy chains. |
| Databáze: | OpenAIRE |
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