Effect of Temperature on the Growth and Cell Wall Chemistry of a Facultative Thermophilic Bacillus
| Autor: | Richard H. Himes, J. M. Akagi, May Chan, Thomas J. Novitsky |
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| Rok vydání: | 1974 |
| Předmět: |
Chromatography
Gas Hot Temperature Physiology and Metabolism Bacillus Microbiology Phosphates Cell wall chemistry.chemical_compound Cell Wall Fluorometry Magnesium Microscopy Phase-Contrast Amino Acids Molecular Biology Soil Microbiology chemistry.chemical_classification Alanine Teichoic acid Staining and Labeling biology Hydrolysis Thermophile Temperature Galactose Amino Sugars Phosphate biology.organism_classification Amino acid Teichoic Acids Microscopy Electron chemistry Biochemistry Spectrophotometry Bacillus coagulans Peptidoglycan Autolysis |
| Zdroj: | Journal of Bacteriology. 117:858-865 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | The morphology and cell wall composition of Bacillus coagulans , a facultative thermophile, were examined as a function of growth temperature. The morphology of the organism varied when it was grown at different temperatures; at 37 C the organism grew as individual cells which increased in length with increasing growth temperature. At 55 C it grew in long chains of cells. Cell wall prepared from cells grown at 37 C contained 44% teichoic acid by weight, whereas cells grown at 55 C contained 29% teichoic acid. Teichoic acid from these cells was a polymer of glycerol phosphate containing galactose and ester alanine. The ratio of ester alanine to phosphate was significantly higher in cell walls and teichoic acid from 37 C-grown cells compared with those from 55 C-grown cells. Other differences observed were that cells grown at 55 C contained a lower level of autolytic ability, produced cell walls which bound more Mg 2+ , and contained less peptide cross-bridging in its peptidoglycan layer than cells grown at 37 C. |
| Databáze: | OpenAIRE |
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