The clonal origins of dysplasia from intestinal metaplasia in the human stomach
| Autor: | Nicholas A. Wright, Marco Novelli, Lydia Gutierrez–Gonzalez, Eiji Yamada, Manuel Rodriguez–Justo, David L. Stoker, Stuart McDonald, Alicia Green, Simon J. Leedham, Yuuki Kishi, Shigeki Bamba, Janusz Jankowski, I Mitchell, Sean L. Preston, Rebecca Harrison, Tania Ventayol Garcia, Trevor A. Graham |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Loss of Heterozygosity Biology Adenocarcinoma Somatic evolution in cancer DNA Mitochondrial Loss of heterozygosity Electron Transport Complex IV Stomach Neoplasms Metaplasia Gastric glands medicine Humans Laser capture microdissection Aged Hepatology Gastroenterology Intestinal metaplasia Genetic Variation Middle Aged medicine.disease Founder Effect Clone Cells Gene Expression Regulation Neoplastic medicine.anatomical_structure Dysplasia Gastric Mucosa Disease Progression Field cancerization medicine.symptom Cell Division |
| Zdroj: | Gastroenterology. 140(4) |
| ISSN: | 1528-0012 |
| Popis: | Background & Aims Studies of the clonal architecture of gastric glands with intestinal metaplasia are important in our understanding of the progression from metaplasia to dysplasia. It is not clear if dysplasias are derived from intestinal metaplasia or how dysplasias expand. We investigated whether cells within a metaplastic gland share a common origin, whether glands clonally expand by fission, and determine if such metaplastic glands are genetically related to the associated dysplasia. We also examined the clonal architecture of entire dysplastic lesions and the genetic changes associated with progression within dysplasia. Methods Cytochrome c oxidase-deficient (CCO−) metaplastic glands were identified using a dual enzyme histochemical assay. Clonality was assessed by laser capture of multiple cells throughout CCO− glands and polymerase chain reaction sequencing of the entire mitochondrial DNA (mtDNA) genome. Nuclear DNA abnormalities in individual glands were identified by laser capture microdissection polymerase chain reaction sequencing for mutation hot spots and microsatellite loss of heterozygosity analysis. Results Metaplastic glands were derived from the same clone—all lineages shared a common mtDNA mutation. Mutated glands were found in patches that had developed through gland fission. Metaplastic and dysplastic glands can be genetically related, indicating the clonal origin of dysplasia from metaplasia. Entire dysplastic fields contained a founder mutation from which multiple, distinct subclones developed. Conclusions There is evidence for a distinct clonal evolution from metaplasia to dysplasia in the human stomach. By field cancerization, a single clone can expand to form an entire dysplastic lesion. Over time, this field appears to become genetically diverse, indicating that gastric cancer can arise from a subclone of the founder mutation. |
| Databáze: | OpenAIRE |
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