Quantification of Proline-containing Cyclic Dipeptides by LC-MS/MS

Autor:	Yuuki Otsuka, Tomoko Shimamura, Michio Sakaji, Hikaru Arita, Hiroyuki Ukeda, Takehiro Kashiwagi
Rok vydání:	2020
Předmět:	Taste Chromatography Proline Chemistry food and beverages Peptides Cyclic Analytical Chemistry Limit of Detection Tandem Mass Spectrometry Lc ms ms Fermentation Fermentation in food processing Quantitative analysis (chemistry) Chromatography Liquid
Zdroj:	Analytical Sciences. 36:977-980
ISSN:	1348-2246 0910-6340
Popis:	The cyclic dipeptides, 2,5-diketopiperazines (DKPs), draw attention as bioactive and taste compounds. DKPs, especially those containing Proline (Pro), are contained in heated and fermented foods. Herein, we developed a method for a simultaneous quantitative analysis of Pro-containing DKPs using LC-MS/MS. After optimizing the LC-MS/MS conditions, the developed method was applied to quantify Pro-containing DKPs in Goishi tea, which is a post-fermented tea produced by a two-step aerobic-anaerobic fermentation process. Consequently, 17 kinds of Pro-containing DKPs could be quantified, and the total amount of Pro-containing DKPs was 3.40 mg/L. The recovery in a spiked test was 93 - 117%, which is satisfactory. We believe that the developed method can be used to elucidate the role of Pro-containing DKPs in food.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c3dd5096ad4b19c3d38aadf423f8725 https://doi.org/10.2116/analsci.19p470 Zobrazit plný text záznamu Full text from SpringerLink