The Timing oflin-4RNA Accumulation Controls the Timing of Postembryonic Developmental Events inCaenorhabditis elegans
| Autor: | Victor R. Ambros, Rhonda L. Feinbaum |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Small RNA
heterochronic genes Transgene Down-Regulation Helminth genetics Animals Genetically Modified Protein biosynthesis Animals RNA Messenger Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Molecular Biology Genes Helminth Regulation of gene expression biology C. elegans fungi Gene Expression Regulation Developmental Nuclear Proteins RNA Helminth Proteins Cell Biology biology.organism_classification Immunohistochemistry Molecular biology lin-4RNA Phenotype Protein Biosynthesis RNA Helminth LIN-14 developmental timing Developmental Biology |
| Zdroj: | Developmental Biology. 210(1):87-95 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1999.9272 |
| Popis: | The lin-4 gene encodes a small RNA that is required to translationally repress lin-14 toward the end of the first larval stage of Caenorhabditis elegans development. To determine if the timing of LIN-14 protein down-regulation depends on the temporal profile of lin-4 RNA level, we analyzed the stage-specificity of lin-4 RNA expression during wild-type development and examined the phenotypes of transgenic worms that overexpress lin-4 RNA during the first larval stage. We found that lin-4 RNA first becomes detectable at approximately 12 h of wild-type larval development and rapidly accumulates to nearly maximum levels by 16 h. This profile of lin-4 RNA accumulation corresponded to the timing of LIN-14 protein down-regulation. Transgenic strains that express elevated levels of lin-4 RNA prior to 12 h of development display reduced levels of LIN-14 protein and precocious phenotypes consistent with abnormally early loss of lin-14 activity. These results indicate that the temporal profile of lin-4 RNA accumulation specifies the timing of LIN-14 down-regulation and thereby controls the timing of postembryonic developmental events. |
| Databáze: | OpenAIRE |
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