Methicillin-resistant Staphylococcus aureus genotyping using a small set of polymorphisms
Autor: | Wendy J. Munckhof, Graeme R. Nimmo, Alex J. Stephens, John Inman-Bamber, Erin P. Price, Flavia Huygens, Jacqueline Schooneveldt, Philip M. Giffard |
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Rok vydání: | 2006 |
Předmět: |
Microbiology (medical)
Staphylococcus aureus 110000 MEDICAL AND HEALTH SCIENCES Genotype Molecular Sequence Data Single-nucleotide polymorphism MRSA 110802 Medical Infection Agents (incl. Prions) Biology medicine.disease_cause Polymerase Chain Reaction Polymorphism Single Nucleotide Microbiology 080300 COMPUTER SOFTWARE 060501 Bacteriology Bacterial Proteins 080399 Computer Software not elsewhere classified medicine Humans SNP Genotyping 060502 Infectious Agents Genetics 100499 Medical Biotechnology not elsewhere classified Base Sequence 100402 Medical Biotechnology Diagnostics (incl. Biosensors) 060503 Microbial Genetics SCCmec Australia 060500 MICROBIOLOGY General Medicine Methicillin-resistant Staphylococcus aureus Subtyping Bacterial Typing Techniques 110800 MEDICAL MICROBIOLOGY SNP genotyping 060000 BIOLOGICAL SCIENCES time PCR genotyping Trans-Activators Multilocus sequence typing Methicillin Resistance 110801 Medical Bacteriology SNPs Real |
Zdroj: | Journal of Medical Microbiology |
ISSN: | 1473-5644 0022-2615 |
Popis: | The aim of this study was to identify a set of genetic polymorphisms that efficiently divides methicillin-resistant Staphylococcus aureus (MRSA) strains into groups consistent with the population structure. The rationale was that such polymorphisms could underpin rapid real-time PCR or low-density array-based methods for monitoring MRSA dissemination in a cost-effective manner. Previously, the authors devised a computerized method for identifying sets of single nucleotide polymorphisms (SNPs) with high resolving power that are defined by multilocus sequence typing (MLST) databases, and also developed a real-time PCR method for interrogating a seven-member SNP set for genotyping S. aureus. Here, it is shown that these seven SNPs efficiently resolve the major MRSA lineages and define 27 genotypes. The SNP-based genotypes are consistent with the MRSA population structure as defined by eburst analysis. The capacity of binary markers to improve resolution was tested using 107 diverse MRSA isolates of Australian origin that encompass nine SNP-based genotypes. The addition of the virulence-associated genes cna, pvl and bbp/sdrE, and the integrated plasmids pT181, pI258 and pUB110, resolved the nine SNP-based genotypes into 21 combinatorial genotypes. Subtyping of the SCCmec locus revealed new SCCmec types and increased the number of combinatorial genotypes to 24. It was concluded that these polymorphisms provide a facile means of assigning MRSA isolates into well-recognized lineages. |
Databáze: | OpenAIRE |
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