MicroRNA-137 Inhibited Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells by Targeting Calpain-2
| Autor: | Hong Liu, Jie Qiao, Xiao-Yue Ge, Mao-Zhong Yao, Weifang Zhang, Qian Wu, Chang-Ping Hu, Tiantian Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Article Subject Hypertension Pulmonary Myocytes Smooth Muscle Pulmonary Artery Vascular Remodeling Muscle Smooth Vascular General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley Downregulation and upregulation Cell Movement medicine.artery microRNA medicine Animals Hypoxia Cells Cultured Cell Proliferation Gene knockdown General Immunology and Microbiology Calpain Chemistry Cell growth General Medicine Hypoxia (medical) medicine.disease Pulmonary hypertension Cell Hypoxia Rats MicroRNAs Pulmonary artery Cancer research Calpain-2 Medicine medicine.symptom Research Article |
| Zdroj: | BioMed Research International, Vol 2021 (2021) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2021/2202888 |
| Popis: | The proliferation of pulmonary artery smooth muscle cells (PASMCs) is an important cause of pulmonary vascular remodeling in pulmonary hypertension (PH). It has been reported that miR-137 inhibits the proliferation of tumor cells. However, whether miR-137 is involved in PH remains unclear. In this study, male Sprague-Dawley rats were subjected to 10% O2 for 3 weeks to establish PH, and rat primary PASMCs were treated with hypoxia (3% O2) for 48 h to induce cell proliferation. The effect of miR-137 on PASMC proliferation and calpain-2 expression was assessed by transfecting miR-137 mimic and inhibitor. The effect of calpain-2 on PASMC proliferation was assessed by transfecting calpain-2 siRNA. The present study found for the first time that miR-137 was downregulated in pulmonary arteries of hypoxic PH rats and in hypoxia-treated PASMCs. miR-137 mimic inhibited hypoxia-induced PASMC proliferation and upregulation of calpain-2 expression in PASMCs. Furthermore, miR-137 inhibitor induced the proliferation of PASMCs under normoxia, and knockdown of calpain-2 mRNA by siRNA significantly inhibited hypoxia-induced proliferation of PASMCs. Our study demonstrated that hypoxia-induced downregulation of miR-137 expression promoted the proliferation of PASMCs by targeting calpain-2, thereby potentially resulting in pulmonary vascular remodeling in hypoxic PH. |
| Databáze: | OpenAIRE |
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