Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy
| Autor: | Hoon Jai Chun, Man So Han, Seung Taek Hong, Bong Rae Cho, Kyu Cheol Paik, Seong Jun Park, Sung An Kwon, Tae Hyeong Kim, Ji Woo Choi, Jung Nyoung Heo, Eun Sun Kim |
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| Rok vydání: | 2017 |
| Předmět: |
Absorption (pharmacology)
Tris 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Analytical Chemistry chemistry.chemical_compound Two-photon excitation microscopy Humans Fluorescent Dyes Photons Molecular Structure Chemistry Vesicle Hep G2 Cells Hydrogen-Ion Concentration HCT116 Cells 021001 nanoscience & nanotechnology Molecular biology 0104 chemical sciences Human colon cancer Microscopy Fluorescence Multiphoton Biochemistry Cell culture Colonic Neoplasms Hepatic stellate cell 0210 nano-technology Citric acid |
| Zdroj: | Analytical Chemistry. 89:9830-9835 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.7b01804 |
| Popis: | We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10–50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating... |
| Databáze: | OpenAIRE |
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