Activated platelets in central venous catheters: a flow cytometry and numerical simulation approach
| Autor: | Jonathas Haniel, Luara Isabela dos Santos, Rudolf Huebner, Maria das Graças Carvalho, Thabata Coaglio Lucas, Sissy Araújo Trindade, Marcelo Henrique Fernandes Ottoni, Rita Carolina Figueiredo Duarte |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
medicine.diagnostic_test Computer simulation renal dialysis vascular access devices coagulation agents biomarkers flow mechanics polymer interaction Chemistry General Medicine General Biochemistry Genetics and Molecular Biology Flow cytometry RS1-441 R5-920 Pharmacy and materia medica medicine Platelet activation Biomedical engineering |
| Zdroj: | Acta Scientiarum. Health Sciences, Vol 43, Iss 1 (2021) |
| ISSN: | 1807-8648 1679-9291 0029-1072 |
| Popis: | The central venous catheter that is inserted in patients undergoing hemodialysis can cause hemodynamic instability and trigger complications such as thrombus formation. The objective of this study was to investigate hemostatic and numerical influences on thrombus formation in patients undergoing hemodialysis with a central venous catheter. Participants were assigned to three groups: I: clinical and laboratorial healthy individuals matched by sex and age (controls); II: participants after one month of insertion of the catheter and III: participants after 4 months of insertion of the catheter. Platelet activation was investigated by GPIIb/IIIa and p-selectin expressions using flow cytometry. A three-dimensional model of the catheter was constructed in the numerical simulation for the calculation of partial differential equation of a platelet activation model. A significant difference was detected by the expression of p-selectin comparing the group I (33.42 ± 4.74), group II (40.79 ± 5.54) and group III(51.00 ± 7.21) (p < 0.0001). The median values for GPIIb/IIIa were 10426 (10029-10721), 13921 (13412-15652) and 19946 (18714-21815) after catheter insertion (p < 0.0001), for groups I, II and III, respectively. Excluding the first arterial orifice, venous orifices tend to have greater platelet activation when compared to the other arterial orifices. The results of this study showed the influence of arterial and venous lateral orifices in stimulating the development of thrombi associated with the activation of platelet markers the longer the catheter was used |
| Databáze: | OpenAIRE |
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