Expression and cellular localization of prolactin and the prolactin receptor in mammalian retina
| Autor: | Stéphanie Thebault, Mayda Ramírez, Gabriel Nava, Gonzalo Martínez de la Escalera, José Carlos Rivera, Carmen Clapp, Jorge Aranda, Fernando López-Barrera, Juan Riesgo |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Retinal Ganglion Cells endocrine system Receptors Prolactin In situ hybridization Retina Cellular and Molecular Neuroscience Immunolabeling Species Specificity Interneurons Chlorocebus aethiops medicine Animals RNA Messenger Rats Wistar Ganglion cell layer In Situ Hybridization Cellular localization Mammals Glial fibrillary acidic protein biology Reverse Transcriptase Polymerase Chain Reaction Prolactin receptor Molecular biology Sensory Systems Prolactin Rats Ophthalmology medicine.anatomical_structure Astrocytes Inner nuclear layer biology.protein sense organs hormones hormone substitutes and hormone antagonists Photoreceptor Cells Vertebrate |
| Zdroj: | Experimental Eye Research. 86:314-321 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2007.11.003 |
| Popis: | Prolactin (PRL), originally associated with milk secretion, is known to have a wide variety of biological actions and diverse sites of production beyond the pituitary gland. Recent studies have demonstrated that PRL is synthesized in retinal tissue. To gain insights into the functional role of PRL in the mammalian retina, we mapped the distribution of the PRL protein and the expression and localization of the PRL receptor (PRLR) in the retina of adult rats and green monkeys. PRL was examined in retinal sections by double immunolabeling combining anti-PRL antibodies with antibodies specific for glutamine synthetase (labeling Muller cells), glial fibrillary acidic protein (labeling astrocytes), or neuronal nuclei protein (labeling neurons). PRL was detected throughout the rat retina: in the photoreceptor outer segments, Muller cells, interneurons, ganglion cells, and astrocytes. The PRLR was examined by RT–PCR, in situ hybridization, immunohistochemistry, and Western blot. The long isoform of the PRLR was localized in the photoreceptor nuclear layer, inner nuclear layer, and ganglion cell layer of rat retina. The monkey retina showed a similar distribution of PRL and PRLR immunoreactivities. These findings suggest that PRL functions as a local regulator of various cell types in the mammalian retina. |
| Databáze: | OpenAIRE |
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