Comammox Nitrospira are the dominant ammonia oxidizers in a mainstream low dissolved oxygen nitrification reactor
| Autor: | James S. Griffin, Joseph A. Kozak, Fabrizio Sabba, Fenghua Yang, Yubo Wang, Alex Rosenthal, George Wells, Heng Zhang, Paul Roots, Morgan L. Petrovich |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
0208 environmental biotechnology Sequencing batch reactor 02 engineering and technology 010501 environmental sciences 01 natural sciences 03 medical and health sciences Ammonia chemistry.chemical_compound Nitrate RNA Ribosomal 16S Waste Management and Disposal Phylogeny 030304 developmental biology 0105 earth and related environmental sciences Water Science and Technology Civil and Structural Engineering 0303 health sciences Bacteria biology Ecological Modeling Comammox biology.organism_classification Nitrification Pollution 6. Clean water 020801 environmental engineering Oxygen Activated sludge chemistry Wastewater Environmental chemistry Sewage treatment Oxidation-Reduction Nitrospira |
| Zdroj: | Water Research. 157:396-405 |
| ISSN: | 0043-1354 |
| DOI: | 10.1016/j.watres.2019.03.060 |
| Popis: | Recent findings show that a subset of bacteria affiliated withNitrospira, a genus known for its importance in nitrite oxidation for biological nutrient removal applications, are capable ofcompleteammoniaoxidation (comammox) to nitrate. Early reports suggested that they were absent or present in low abundance in most activated sludge processes, and thus likely functionally irrelevant. Here we show the accumulation of comammoxNitrospirain a nitrifying sequencing batch reactor operated at low dissolved oxygen (DO) concentrations. Actual mainstream wastewater was used as influent after primary settling and an upstream pre-treatment process for carbon and phosphorus removal. The ammonia removal rate was stable and exceeded that of the treatment plant’s parallel full-scale high DO nitrifying activated sludge reactor. 16S rRNA sequencing showed a steady accumulation ofNitrospirato 53% total abundance and a decline in conventional ammonia oxidizing bacteria to Nitrospirabeginning around day 200, to eventually comprise 94% of all detectedamoAand 4% of total bacteria by day 407. Quantitative fluorescence in-situ hybridization confirmed the increasing trend and high relative abundance ofNitrospira. These results demonstrate that comammox can be metabolically relevant to nitrogen transformation in wastewater treatment, and can even dominate the ammonia oxidizing community. Our results suggest that comammox may be an important functional group in energy efficient nitrification systems designed to operate at low DO levels. |
| Databáze: | OpenAIRE |
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