Enhanced Antimicrobial and Anticancer Activity of Silver and Gold Nanoparticles Synthesised Using Sargassum incisifolium Aqueous Extracts
| Autor: | Mervin Meyer, Nicole Remaliah Samantha Sibuyi, Denzil R. Beukes, Kim Durrell, Edith Antunes, Marilize Le Roes-Hill, Mokone Mmola, John J. Bolton |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Acinetobacter baumannii
Dispersity Pharmaceutical Science Nanoparticle Metal Nanoparticles 02 engineering and technology 01 natural sciences Silver nanoparticle Analytical Chemistry antibacterial activity Drug Resistance Multiple Bacterial Drug Discovery Candida albicans Zeta potential Enterococcus faecalis Organic chemistry Aqueous solution Chemistry green synthesis 021001 nanoscience & nanotechnology Anti-Bacterial Agents Klebsiella pneumoniae Chemistry (miscellaneous) Colloidal gold total phenolic content MCF-7 Cells Molecular Medicine cytotoxicity 0210 nano-technology Antibacterial activity HT29 Cells Methicillin-Resistant Staphylococcus aureus silver nanoparticles Silver Antineoplastic Agents Microbial Sensitivity Tests 010402 general chemistry Article gold nanoparticles fucoidans Sargassum incisifolium lcsh:QD241-441 lcsh:Organic chemistry Microscopy Electron Transmission Cell Line Tumor Humans Physical and Theoretical Chemistry Particle Size Plant Extracts Organic Chemistry Sargassum Spectrometry X-Ray Emission Green Chemistry Technology 0104 chemical sciences Polyphenol Gold Nuclear chemistry |
| Zdroj: | Molecules Molecules, Vol 21, Iss 12, p 1633 (2016) Molecules; Volume 21; Issue 12; Pages: 1633 |
| ISSN: | 1420-3049 |
| Popis: | A detailed, methodical approach was used to synthesise silver and gold nanoparticles using two differently prepared aqueous extracts of the brown algae Sargassum incisifolium. The efficiency of the extracts in producing nanoparticles were compared to commercially available brown algal fucoidans, a major constituent of brown algal aqueous extracts. The nanoparticles were characterised using TEM, XRD and UV/Vis spectroscopy and zeta potential measurements. The rate of nanoparticle formation was assessed using UV/Vis spectroscopy and related to the size, shape and morphology of the nanoparticles as revealed by TEM. The antioxidant, reducing power and total polyphenolic contents of the aqueous extracts and fucoidans were determined, revealing that the aqueous extracts with the highest contents produced smaller, spherical, more monodisperse nanoparticles at a faster rate. The nanoparticles were assessed against two gram-negative bacteria, two gram-positive bacteria and one yeast strain. In contrast to the literature, the silver nanoparticles produced using the aqueous extracts were particularly toxic to Gram-negative bacteria, while the gold nanoparticles lacked activity. The cytotoxic activity of the nanoparticles was also evaluated against cancerous (HT-29, MCF-7) and non-cancerous (MCF-12a) cell lines. The silver nanoparticles displayed selectivity, since the MCF-12a cell line was found to be resistant to the nanoparticles, while the cancerous HT-29 cell line was found to be sensitive (10% viability). The gold nanoparticles displayed negligible toxicity. |
| Databáze: | OpenAIRE |
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