Low dopamine transporter mRNA levels in midbrain regions containing calbindin
| Autor: | Anthony M. lacopino, Krebreten F. Manaye, Dwight C. German, Manjit K. Sanghera, Michael J. Bannon, C. L. Liang |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Calbindins medicine.medical_specialty Tyrosine 3-Monooxygenase Gene Expression Nerve Tissue Proteins In situ hybridization Sulfur Radioisotopes Calbindin Midbrain Mice chemistry.chemical_compound S100 Calcium Binding Protein G Mesencephalon Dopamine Internal medicine medicine Animals Humans Neurotoxin RNA Messenger In Situ Hybridization Dopamine transporter Dopamine Plasma Membrane Transport Proteins Membrane Glycoproteins biology Chemistry General Neuroscience MPTP Dopaminergic Membrane Transport Proteins Immunohistochemistry Molecular biology Rats nervous system diseases Mice Inbred C57BL Endocrinology nervous system Calbindin 1 biology.protein Carrier Proteins medicine.drug |
| Zdroj: | NeuroReport. 5:1641-1644 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-199408150-00025 |
| Popis: | The dopamine transporter (DAT) is the site at which the neurotoxic metabolite of MPTP gains access to midbrain dopaminergic (DA) neurons. However, not all midbrain DA neurons degenerate following MPTP treatment. The midbrain DA neurons that contain the calcium-binding protein, calbindin-D28k (CALB), are relatively invulnerable to MPTP toxicity, compared with DA neurons that lack CALB. Using in situ hybridization and immunocytochemical staining techniques in the rat and mouse, we now report that there is as much as 10 fold less DAT mRNA in regions where DA neurons contain CALB compared with regions where DA neurons lack CALB. These data suggest that specific midbrain DA neurons are invulnerable to MPTP toxicity not only because they contain CALB, but also because they have relatively low DAT activity. |
| Databáze: | OpenAIRE |
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