Corneocyte lysis and fragmented DNA considerations for the cellular component of forensic touch DNA
Autor: | Elli Rammenou, Julia Burrill, Fatima Alawar, Barbara Daniel, Nunzianda Frascione |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Keratinocytes Lysis Touch DNA Cell Separation DNA Fragmentation Polymerase Chain Reaction Pathology and Forensic Medicine Cornified envelope 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics Fluorescence microscope Sample Type Humans 030216 legal & forensic medicine Corneocyte DNA Degradation Necrotic DNA DNA Fingerprinting Cell biology 030104 developmental biology chemistry Microscopy Fluorescence Touch Cellular component Epidermis Microsatellite Repeats |
Zdroj: | Forensic science international. Genetics. 51 |
ISSN: | 1878-0326 |
Popis: | DNA deposited by individuals' hands is a routine part of forensic analysis, yet little is understood about the precise cellular contents left by handling. "Dead" skin cells known as corneocytes make up the majority of the cellular material left in touch deposits by people's hands but are known to lack nuclei, making their DNA content ambiguous. Here we measure DNA released from anucleate corneocytes following various lysis methods to determine how much DNA may be present in these cells and how best to recover it from inside the cornified envelope. We demonstrate that enhanced lysis methods using a reducing agent and longer incubation may be valuable for hand deposit samples. Corneocyte DNA can be characterized as highly degraded based on the quantification, STR profiling and fluorescence microscopy of the cells from freshly washed hands. Purification to target shorter DNA fragments is demonstrated. DNA from the washed corneocyte cells is shown to constitute the majority of recoverable DNA with these methods. We consider the use of new methods adapted to cornified cells and fragmented DNA for future research into this sample type. |
Databáze: | OpenAIRE |
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